Neutral Sphingomyelinase Modulation in the Protective/Preventive Role of rMnSOD from Radiation-Induced Damage in the Brain
Abstract
:1. Introduction
2. Results
2.1. Protective and Preventive Effect of rMnSOD on Radiation-Induced Structural Changes in Midbrain Tissue
2.2. nSMase Is Required for the Protective and Preventive Effect of rMnSOD
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Experimental Design and Animal Care
4.3. Morphological and Immunohistochemistry Analysis
4.4. Reverse Transcription Quantitative PCR (RTqPCR)
4.5. Protein Concentration and Western Blotting
4.6. nSMaseActivity Assay
4.7. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Cataldi, S.; Borrelli, A.; Ceccarini, M.R.; Nakashidze, I.; Codini, M.; Belov, O.; Ivanov, A.; Krasavin, E.; Ferri, I.; Conte, C.; et al. Neutral Sphingomyelinase Modulation in the Protective/Preventive Role of rMnSOD from Radiation-Induced Damage in the Brain. Int. J. Mol. Sci. 2019, 20, 5431. https://doi.org/10.3390/ijms20215431
Cataldi S, Borrelli A, Ceccarini MR, Nakashidze I, Codini M, Belov O, Ivanov A, Krasavin E, Ferri I, Conte C, et al. Neutral Sphingomyelinase Modulation in the Protective/Preventive Role of rMnSOD from Radiation-Induced Damage in the Brain. International Journal of Molecular Sciences. 2019; 20(21):5431. https://doi.org/10.3390/ijms20215431
Chicago/Turabian StyleCataldi, Samuela, Antonella Borrelli, Maria Rachele Ceccarini, Irina Nakashidze, Michela Codini, Oleg Belov, Alexander Ivanov, Eugene Krasavin, Ivana Ferri, Carmela Conte, and et al. 2019. "Neutral Sphingomyelinase Modulation in the Protective/Preventive Role of rMnSOD from Radiation-Induced Damage in the Brain" International Journal of Molecular Sciences 20, no. 21: 5431. https://doi.org/10.3390/ijms20215431