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Open AccessArticle

Organ-Specific Analysis of Morus alba Using a Gel-Free/Label-Free Proteomic Technique

1
College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, China
2
Faculty of Environmental and Information Sciences, Fukui University of Technology, Fukui 910-8505, Japan
3
Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 310027, China
4
Key Laboratory for Biomedical Engineering of Ministry of Education, Zhejiang-Malaysia Joint Research Center for Traditional Medicine, Zhejiang University, Hangzhou 310027, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(2), 365; https://doi.org/10.3390/ijms20020365
Received: 15 December 2018 / Revised: 14 January 2019 / Accepted: 15 January 2019 / Published: 16 January 2019
(This article belongs to the Special Issue Plant Proteomic Research 2.0)
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PDF [5467 KB, uploaded 16 January 2019]
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Abstract

Morus alba is an important medicinal plant that is used to treat human diseases. The leaf, branch, and root of Morus can be applied as antidiabetic, antioxidant, and anti-inflammatory medicines, respectively. To explore the molecular mechanisms underlying the various pharmacological functions within different parts of Morus, organ-specific proteomics were performed. Protein profiles of the Morus leaf, branch, and root were determined using a gel-free/label-free proteomic technique. In the Morus leaf, branch, and root, a total of 492, 414, and 355 proteins were identified, respectively, including 84 common proteins. In leaf, the main function was related to protein degradation, photosynthesis, and redox ascorbate/glutathione metabolism. In branch, the main function was related to protein synthesis/degradation, stress, and redox ascorbate/glutathione metabolism. In root, the main function was related to protein synthesis/degradation, stress, and cell wall. Additionally, organ-specific metabolites and antioxidant activities were analyzed. These results revealed that flavonoids were highly accumulated in Morus root compared with the branch and leaf. Accordingly, two root-specific proteins named chalcone flavanone isomerase and flavonoid 3,5-hydroxylase were accumulated in the flavonoid pathway. Consistent with this finding, the content of the total flavonoids was higher in root compared to those detected in branch and leaf. These results suggest that the flavonoids in Morus root might be responsible for its biological activity and the root is the main part for flavonoid biosynthesis in Morus. View Full-Text
Keywords: Morus; organ; gel-free/label-free proteomics; flavonoid; antioxidant activity Morus; organ; gel-free/label-free proteomics; flavonoid; antioxidant activity
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Zhu, W.; Zhong, Z.; Liu, S.; Yang, B.; Komatsu, S.; Ge, Z.; Tian, J. Organ-Specific Analysis of Morus alba Using a Gel-Free/Label-Free Proteomic Technique. Int. J. Mol. Sci. 2019, 20, 365.

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