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Open AccessArticle

Castration Induces Down-Regulation of A-Type K+ Channel in Rat Vas Deferens Smooth Muscle

1
Department of Pharmacology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Japan
2
Department of Molecular and Cellular Pharmacology, Graduate School of Pharmacological Sciences, Nagoya City University, Nagoya 467-8603, Japan
3
Laboratory of Cellular Pharmacology, Aichi Gakuin University, Nagoya 464-8650, Japan
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(17), 4073; https://doi.org/10.3390/ijms20174073
Received: 4 July 2019 / Revised: 3 August 2019 / Accepted: 19 August 2019 / Published: 21 August 2019
(This article belongs to the Special Issue Ion Channel and Ion-Related Signaling 2019)
A-type K+ channels contribute to regulating the propagation and frequency of action potentials in smooth muscle cells (SMCs). The present study (i) identified the molecular components of A-type K+ channels in rat vas deferens SMs (VDSMs) and (ii) showed the long-term, genomic effects of testosterone on their expression in VDSMs. Transcripts of the A-type K+ channel α subunit, Kv4.3L and its regulatory β subunits, KChIP3, NCS1, and DPP6-S were predominantly expressed in rat VDSMs over the other related subtypes (Kv4.2, KChIP1, KChIP2, KChIP4, and DPP10). A-type K+ current (IA) density in VDSM cells (VDSMCs) was decreased by castration without changes in IA kinetics, and decreased IA density was compensated for by an oral treatment with 17α-methyltestosterone (MET). Correspondingly, in the VDSMs of castrated rats, Kv4.3L and KChIP3 were down-regulated at both the transcript and protein expression levels. Changes in Kv4.3L and KChIP3 expression levels were compensated for by the treatment with MET. These results suggest that testosterone level changes in testosterone disorders and growth processes control the functional expression of A-type K+ channels in VDSMCs. View Full-Text
Keywords: A-type K+ channel; KV4.3; castration; testosterone; vas deferens; smooth muscle A-type K+ channel; KV4.3; castration; testosterone; vas deferens; smooth muscle
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MDPI and ACS Style

Ohya, S.; Ito, K.; Hatano, N.; Ohno, A.; Muraki, K.; Imaizumi, Y. Castration Induces Down-Regulation of A-Type K+ Channel in Rat Vas Deferens Smooth Muscle. Int. J. Mol. Sci. 2019, 20, 4073.

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