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Open AccessArticle

Tobacco Cutworm (Spodoptera Litura) Larvae Silenced in the NADPH-Cytochrome P450 Reductase Gene Show Increased Susceptibility to Phoxim

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Guangdong Province Key Laboratory of Eco-Circular Agriculture, South China Agricultural University, Guangzhou 510642, China
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Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China
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State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, East Campus, Guangzhou 510006, China
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New Rural Development Research Institute of South China Agricultural University, Guangzhou 510642, China
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Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(15), 3839; https://doi.org/10.3390/ijms20153839
Received: 21 June 2019 / Revised: 28 July 2019 / Accepted: 5 August 2019 / Published: 6 August 2019
Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductases (CPRs) function as redox partners of cytochrome P450 monooxygenases (P450s). CPRs and P450s in insects have been found to participate in insecticide resistance. However, the CPR of the moth Spodoptera litura has not been well characterized yet. Based on previously obtained transcriptome information, a full-length CPR cDNA of S. litura (SlCPR) was PCR-cloned. The deduced amino acid sequence contains domains and residues predicted to be essential for CPR function. Phylogenetic analysis with insect CPR amino acid sequences showed that SlCPR is closely related to CPRs of Lepidoptera. Quantitative reverse transcriptase PCR (RT-qPCR) was used to determine expression levels of SlCPR in different developmental stages and tissues of S. litura. SlCPR expression was strongest at the sixth-instar larvae stage and fifth-instar larvae showed highest expression in the midgut. Expression of SlCPR in the midgut and fat body was strongly upregulated when fifth-instar larvae were exposed to phoxim at LC15 (4 μg/mL) and LC50 (20 μg/mL) doses. RNA interference (RNAi) mediated silencing of SlCPR increased larval mortality by 34.6% (LC15 dose) and 53.5% (LC50 dose). Our results provide key information on the SlCPR gene and indicate that SlCPR expression levels in S. litura larvae influence their susceptibility to phoxim and possibly other insecticides. View Full-Text
Keywords: Spodoptera litura; NADPH-cytochrome; P450 reductase; RNAi; insecticide susceptibility; phoxim Spodoptera litura; NADPH-cytochrome; P450 reductase; RNAi; insecticide susceptibility; phoxim
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Ji, H.-Y.; Staehelin, C.; Jiang, Y.-P.; Liu, S.-W.; Ma, Z.-H.; Su, Y.-J.; Zhang, J.-E.; Wang, R.-L. Tobacco Cutworm (Spodoptera Litura) Larvae Silenced in the NADPH-Cytochrome P450 Reductase Gene Show Increased Susceptibility to Phoxim. Int. J. Mol. Sci. 2019, 20, 3839.

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