Next Article in Journal
Effects of Cholesterol on Water Permittivity of Biomimetic Ion Pair Amphiphile Bilayers: Interplay between Membrane Bending and Molecular Packing
Next Article in Special Issue
In Vitro Entero-Capillary Barrier Exhibits Altered Inflammatory and Exosomal Communication Pattern after Exposure to Silica Nanoparticles
Previous Article in Journal
Renaissance Distribution for Statistically Failed Experiments
Previous Article in Special Issue
The Role of Endothelial Dysfunction in Peripheral Blood Nerve Barrier: Molecular Mechanisms and Pathophysiological Implications
Open AccessArticle

Endothelial Colony Forming Cells as an Autologous Model to Study Endothelial Dysfunction in Patients with a Bicuspid Aortic Valve

1
Department of Cell and Chemical Biology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
2
Department of Cardiology, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands
3
Department of Cardiology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
4
Department of Anatomy and Embryology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(13), 3251; https://doi.org/10.3390/ijms20133251
Received: 6 June 2019 / Revised: 26 June 2019 / Accepted: 1 July 2019 / Published: 2 July 2019
(This article belongs to the Special Issue Endothelial Dysfunction: Pathophysiology and Molecular Mechanisms)
Bicuspid aortic valve (BAV), the most common congenital heart defect, is associated with an increased prevalence of aortic dilation, aortic rupture and aortic valve calcification. Endothelial cells (ECs) play a major role in vessel wall integrity. Little is known regarding EC function in BAV patients due to lack of patient derived primary ECs. Endothelial colony forming cells (ECFCs) have been reported to be a valid surrogate model for several cardiovascular pathologies, thereby facilitating an in vitro system to assess patient-specific endothelial dysfunction. Therefore, the aim of this study was to investigate cellular functions in ECFCs isolated from BAV patients. Outgrowth and proliferation of ECFCs from patients with BAV (n = 34) and controls with a tricuspid aortic valve (TAV, n = 10) were determined and related to patient characteristics. Interestingly, we were only able to generate ECFCs from TAV and BAV patients without aortic dilation, and failed to isolate ECFC colonies from patients with a dilated aorta. Analyzing EC function showed that while proliferation, cell size and endothelial-to-mesenchymal transition were similar in TAV and BAV ECFCs, migration and the wound healing capacity of BAV ECFCs is significantly higher compared to TAV ECFCs. Furthermore, calcification is blunted in BAV compared to TAV ECFCs. Our results reveal ECs dysfunction in BAV patients and future research is required to unravel the underlying mechanisms and to further validate ECFCs as a patient-specific in vitro model for BAV. View Full-Text
Keywords: endothelial colony forming cell; ECFC; blood outgrowth endothelial cell; BOEC; bicuspid aortic valve; BAV; aortic dilation; calcification; migration endothelial colony forming cell; ECFC; blood outgrowth endothelial cell; BOEC; bicuspid aortic valve; BAV; aortic dilation; calcification; migration
Show Figures

Figure 1

MDPI and ACS Style

van de Pol, V.; Bons, L.R.; Lodder, K.; Kurakula, K.B.; Sanchez-Duffhues, G.; Siebelink, H.-M.J.; Roos-Hesselink, J.W.; DeRuiter, M.C.; Goumans, M.-J. Endothelial Colony Forming Cells as an Autologous Model to Study Endothelial Dysfunction in Patients with a Bicuspid Aortic Valve. Int. J. Mol. Sci. 2019, 20, 3251.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop