Next Article in Journal
Effect of Water Chemistry on Antimony Removal by Chemical Coagulation: Implications of ζ-Potential and Size of Precipitates
Previous Article in Journal
Interactions of Glutamatergic Neurotransmission and Brain-Derived Neurotrophic Factor in the Regulation of Behaviors after Nicotine Administration
Previous Article in Special Issue
The Pleiotropic Role of Retinoic Acid/Retinoic Acid Receptors Signaling: From Vitamin A Metabolism to Gene Rearrangements in Acute Promyelocytic Leukemia
Article Menu
Issue 12 (June-2) cover image

Export Article

Open AccessArticle

Chromosomal Evolution and Evolutionary Relationships of Lebiasina Species (Characiformes, Lebiasinidae)

Laboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP 13565-905, Brazil
Secretaria de Estado de Educação de Mato Grosso—SEDUC-MT, Cuiabá, MT 78049-909, Brazil
Facultad de Ciencias Agropecuarias, Universidad Técnica de Machala, Machala 070151, Ecuador
Museu de Zoologia da Universidade de São Paulo (MZUSP), São Paulo, SP 04263-000, Brazil
Department of Genetics and Cytology, Yerevan State University, Yerevan 0063, Armenia
Institute of Human Genetics, University Hospital Jena, 07747 Jena, Germany
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(12), 2944;
Received: 15 May 2019 / Revised: 12 June 2019 / Accepted: 14 June 2019 / Published: 16 June 2019
(This article belongs to the Special Issue Chromosome and Karyotype Variation)
PDF [3429 KB, uploaded 21 June 2019]


We present the first cytogenetic data for Lebiasina bimaculata and L. melanoguttata with the aim of (1) investigating evolutionary events within Lebiasina and their relationships with other Lebiasinidae genera and (2) checking the evolutionary relationships between Lebiasinidae and Ctenoluciidae. Both species have a diploid number 2n = 36 with similar karyotypes and microsatellite distribution patterns but present contrasting C-positive heterochromatin and CMA3+ banding patterns. The remarkable interstitial series of C-positive heterochromatin occurring in L. melanoguttata is absent in L. bimaculata. Accordingly, L. bimaculata shows the ribosomal DNA sites as the only GC-rich (CMA3+) regions, while L. melanoguttata shows evidence of a clear intercalated CMA3+ banding pattern. In addition, the multiple 5S and 18S rDNA sites in L. melanogutatta contrast with single sites present in L. bimaculata. Comparative genomic hybridization (CGH) experiments also revealed a high level of genomic differentiation between both species. A polymorphic state of a conspicuous C-positive, CMA3+, and (CGG)n band was found only to occur in L. bimaculata females, and its possible relationship with a nascent sex chromosome system is discussed. Whole chromosome painting (WCP) and CGH experiments indicate that the Lebiasina species examined and Boulengerella maculata share similar chromosomal sequences, thus supporting the relatedness between them and the evolutionary relationships between the Lebiasinidae and Ctenoluciidae families. View Full-Text
Keywords: fish; karyotype evolution; whole chromosome painting; comparative genomic hybridization fish; karyotype evolution; whole chromosome painting; comparative genomic hybridization

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

de Menezes Cavalcante Sassi, F.; Aguiar de Oliveira, E.; Antonio Carlos Bertollo, L.; Nirchio, M.; Hatanaka, T.; Maria Ferreira Marinho, M.; Moreira-Filho, O.; Aroutiounian, R.; Liehr, T.; B. H. Al-Rikabi, A.; de Bello Cioffi, M. Chromosomal Evolution and Evolutionary Relationships of Lebiasina Species (Characiformes, Lebiasinidae). Int. J. Mol. Sci. 2019, 20, 2944.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top