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Chromosomal Evolution and Evolutionary Relationships of Lebiasina Species (Characiformes, Lebiasinidae)

1
Laboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP 13565-905, Brazil
2
Secretaria de Estado de Educação de Mato Grosso—SEDUC-MT, Cuiabá, MT 78049-909, Brazil
3
Facultad de Ciencias Agropecuarias, Universidad Técnica de Machala, Machala 070151, Ecuador
4
Museu de Zoologia da Universidade de São Paulo (MZUSP), São Paulo, SP 04263-000, Brazil
5
Department of Genetics and Cytology, Yerevan State University, Yerevan 0063, Armenia
6
Institute of Human Genetics, University Hospital Jena, 07747 Jena, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(12), 2944; https://doi.org/10.3390/ijms20122944
Received: 15 May 2019 / Revised: 12 June 2019 / Accepted: 14 June 2019 / Published: 16 June 2019
(This article belongs to the Special Issue Chromosome and Karyotype Variation)
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Abstract

We present the first cytogenetic data for Lebiasina bimaculata and L. melanoguttata with the aim of (1) investigating evolutionary events within Lebiasina and their relationships with other Lebiasinidae genera and (2) checking the evolutionary relationships between Lebiasinidae and Ctenoluciidae. Both species have a diploid number 2n = 36 with similar karyotypes and microsatellite distribution patterns but present contrasting C-positive heterochromatin and CMA3+ banding patterns. The remarkable interstitial series of C-positive heterochromatin occurring in L. melanoguttata is absent in L. bimaculata. Accordingly, L. bimaculata shows the ribosomal DNA sites as the only GC-rich (CMA3+) regions, while L. melanoguttata shows evidence of a clear intercalated CMA3+ banding pattern. In addition, the multiple 5S and 18S rDNA sites in L. melanogutatta contrast with single sites present in L. bimaculata. Comparative genomic hybridization (CGH) experiments also revealed a high level of genomic differentiation between both species. A polymorphic state of a conspicuous C-positive, CMA3+, and (CGG)n band was found only to occur in L. bimaculata females, and its possible relationship with a nascent sex chromosome system is discussed. Whole chromosome painting (WCP) and CGH experiments indicate that the Lebiasina species examined and Boulengerella maculata share similar chromosomal sequences, thus supporting the relatedness between them and the evolutionary relationships between the Lebiasinidae and Ctenoluciidae families. View Full-Text
Keywords: fish; karyotype evolution; whole chromosome painting; comparative genomic hybridization fish; karyotype evolution; whole chromosome painting; comparative genomic hybridization
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de Menezes Cavalcante Sassi, F.; Aguiar de Oliveira, E.; Antonio Carlos Bertollo, L.; Nirchio, M.; Hatanaka, T.; Maria Ferreira Marinho, M.; Moreira-Filho, O.; Aroutiounian, R.; Liehr, T.; B. H. Al-Rikabi, A.; de Bello Cioffi, M. Chromosomal Evolution and Evolutionary Relationships of Lebiasina Species (Characiformes, Lebiasinidae). Int. J. Mol. Sci. 2019, 20, 2944.

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