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Normal and Abortive Buds Transcriptomic Profiling of Broccoli ogu Cytoplasmic Male Sterile Line and Its Maintainer

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, 12 Zhongguancun Nandajie Street, Beijing 100081, China
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Int. J. Mol. Sci. 2018, 19(9), 2501; https://doi.org/10.3390/ijms19092501
Received: 30 June 2018 / Revised: 12 August 2018 / Accepted: 14 August 2018 / Published: 24 August 2018
(This article belongs to the Special Issue Plant Genetics and Molecular Breeding)
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Abstract

Bud abortion is the main factor affecting hybrid seeds’ yield during broccoli cross breeding when using ogura cytoplasmic male sterile (ogu CMS) lines. However, the genes associated with bud abortion are poorly understood. We applied RNA sequencing to analyze the transcriptomes of normal and abortive buds of broccoli maintainer and ogu CMS lines. Functional analysis showed that among the 54,753 annotated unigenes obtained, 74 and 21 differentially expressed genes in common were upregulated and downregulated in ogu CMS abortive buds compared with ogu CMS normal buds, maintainer normal, and abortive buds, respectively. Nineteen of the common differentially expressed genes were enriched by GO terms associated with glycosyl hydrolases, reactive oxygen species scavenging, inhibitor, and protein degradation. Ethylene-responsive transcription factor 115 and transcriptional factor basic helix-loop-helix 137 were significantly upregulated; transcription factors DUO1 and PosF21/RF2a/BZIP34 were downregulated in ogu CMS abortive buds compared with the other groups. Genes related to polygalacturonase metabolism, glycosyl hydrolases, oxidation reduction process, phenylalanine metabolism, and phenylpropanoid biosynthesis were significantly changed in ogu CMS abortive buds. Our results increase our understanding of bud abortion, provide a valuable resource for further functional characterization of ogu CMS during bud abortion, and will aid in future cross breeding of Brassica crops. View Full-Text
Keywords: broccoli; cytoplasmic male sterile; bud abortion; gene expression; transcriptome; RNA-Seq broccoli; cytoplasmic male sterile; bud abortion; gene expression; transcriptome; RNA-Seq
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Shu, J.; Zhang, L.; Liu, Y.; Li, Z.; Fang, Z.; Yang, L.; Zhuang, M.; Zhang, Y.; Lv, H. Normal and Abortive Buds Transcriptomic Profiling of Broccoli ogu Cytoplasmic Male Sterile Line and Its Maintainer. Int. J. Mol. Sci. 2018, 19, 2501.

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