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Int. J. Mol. Sci. 2018, 19(8), 2374; https://doi.org/10.3390/ijms19082374

Copper Binding Features of Tropomyosin-Receptor-Kinase-A Fragment: Clue for Neurotrophic Factors and Metals Link

1
Institute of Biostructures and Bioimages, National Council of Research (CNR), Via Paolo Gaifami 18, 95126 Catania, Italy
2
Consorzio Interuniversitario di Ricerca in Chimica dei Metalli nei Sistemi Biologici (CIRCMSB), via Celso Ulpiani, 27, 70125 Bari, Italy
3
Department of Pharmacy, University of Pisa, Via Bonanno Pisano 6, 56126 Pisa, Italy
*
Author to whom correspondence should be addressed.
Received: 30 June 2018 / Revised: 1 August 2018 / Accepted: 7 August 2018 / Published: 12 August 2018
(This article belongs to the Special Issue Interaction between Metal Compounds and Proteins)
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Abstract

The nerve growth factor (NGF) is a neurotrophin essential for the development and maintenance of neurons, whose activity is influenced by copper ions. The NGF protein exerts its action by binding to its specific receptor, TrkA. In this study, a specific domain of the TrkA receptor, region 58–64, was synthesized and its copper(II) complexes characterized by means of potentiometric and spectroscopic studies. The two vicinal histidine residues provide excellent metal anchoring sites and, at physiological pH, a complex with the involvement of the peptide backbone amide nitrogen is the predominant species. The TrkA peptide is competitive for metal binding with analogous peptides due to the N-terminal domain of NGF. These data provide cues for future exploration of the effect of metal ions on the activity of the NGF and its specific cellular receptor. View Full-Text
Keywords: neurotrophin; copper; metal complexes; peptide; receptor neurotrophin; copper; metal complexes; peptide; receptor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Magrì, A.; La Mendola, D. Copper Binding Features of Tropomyosin-Receptor-Kinase-A Fragment: Clue for Neurotrophic Factors and Metals Link. Int. J. Mol. Sci. 2018, 19, 2374.

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