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Open AccessArticle

Green Tea Polyphenols Coupled with a Bioactive Titanium Alloy Surface: In Vitro Characterization of Osteoinductive Behavior through a KUSA A1 Cell Study

1
Department of Applied Science and Technology, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy
2
Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8126, Japan
3
Department of immunology, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan
4
Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan
5
Department of Molecular Cell Physiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan
6
The Center for Advanced Medical Engineering and Informatics, Osaka University, Yamadaoka, Suita, Osaka 565-0871, Japan
7
Department of Orthopedic Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(8), 2255; https://doi.org/10.3390/ijms19082255
Received: 12 July 2018 / Revised: 24 July 2018 / Accepted: 29 July 2018 / Published: 1 August 2018
A chemically-treated titanium alloy (Ti6Al4V) surface, able to induce hydroxyapatite precipitation from body fluids (inorganic mineralization activity), was functionalized with a polyphenolic extract from green tea (tea polyphenols, TPH). Considering that green tea polyphenols have stimulating effects on bone forming cells (biological mineralization), the aim was to test their osteoinductive behavior due to co-operation of inorganic and biological mineralization on mesenchymal stem cells KUSA A1. The functionalized surfaces were characterized by using the Folin–Ciocalteu method and X-ray photoelectron spectroscopy to confirm the successful outcome of the functionalization process. Two cell cultures of mesenchymal stem cells, KUSA A1 were performed, with or without osteoinductive factors. The cells and surfaces were characterized for monitoring cell viability and hydroxyapatite production: Fourier Transform Infrared Spectroscopy and Raman spectroscopy analyses showed deposition of hydroxyapatite and collagen due to the cell activity, highlighting differentiation of KUSA A1 into osteoblasts. A higher production of extracellular matrix was highlighted on the functionalized samples by laser microscope and the fluorescence images showed higher viability of cells and greater presence of osteocalcin in these samples. These results highlight the ability of polyphenols to improve cell differentiation and to stimulate biological mineralization, showing that surface functionalization of metal implants could be a promising way to improve osteointegrability. View Full-Text
Keywords: titanium alloy; polyphenols; green tea; bioactivity; osteoinduction titanium alloy; polyphenols; green tea; bioactivity; osteoinduction
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MDPI and ACS Style

Cazzola, M.; Ferraris, S.; Boschetto, F.; Rondinella, A.; Marin, E.; Zhu, W.; Pezzotti, G.; Vernè, E.; Spriano, S. Green Tea Polyphenols Coupled with a Bioactive Titanium Alloy Surface: In Vitro Characterization of Osteoinductive Behavior through a KUSA A1 Cell Study. Int. J. Mol. Sci. 2018, 19, 2255. https://doi.org/10.3390/ijms19082255

AMA Style

Cazzola M, Ferraris S, Boschetto F, Rondinella A, Marin E, Zhu W, Pezzotti G, Vernè E, Spriano S. Green Tea Polyphenols Coupled with a Bioactive Titanium Alloy Surface: In Vitro Characterization of Osteoinductive Behavior through a KUSA A1 Cell Study. International Journal of Molecular Sciences. 2018; 19(8):2255. https://doi.org/10.3390/ijms19082255

Chicago/Turabian Style

Cazzola, Martina; Ferraris, Sara; Boschetto, Francesco; Rondinella, Alfredo; Marin, Elia; Zhu, Wenliang; Pezzotti, Giuseppe; Vernè, Enrica; Spriano, Silvia. 2018. "Green Tea Polyphenols Coupled with a Bioactive Titanium Alloy Surface: In Vitro Characterization of Osteoinductive Behavior through a KUSA A1 Cell Study" Int. J. Mol. Sci. 19, no. 8: 2255. https://doi.org/10.3390/ijms19082255

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