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Open AccessArticle

Probing the Effect of Physiological Concentrations of IL-6 on Insulin Secretion by INS-1 832/3 Insulinoma Cells under Diabetic-Like Conditions

1
School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham B15 2TT, UK
2
Institute for Metabolism and Systems Research (IMSR), University of Birmingham, Birmingham B15 2TT, UK
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(7), 1924; https://doi.org/10.3390/ijms19071924
Received: 24 May 2018 / Revised: 18 June 2018 / Accepted: 26 June 2018 / Published: 30 June 2018
(This article belongs to the Special Issue Cell and Molecular Biology of Pancreatic Disorders)
Exercise improves insulin secretion by pancreatic beta cells (β-cells) in patients with type 2 diabetes, but molecular mechanisms of this effect are yet to be determined. Given that contracting skeletal muscle causes a spike in circulating interleukin-6 (IL-6) levels during exercise, muscle-derived IL-6 is a possible endocrine signal associated with skeletal muscle to β-cell crosstalk. Evidence to support a role of IL-6 in regulating the health and function of β-cells is currently inconsistent and studies investigating the role of IL-6 on the function of β-cells exposed to type 2 diabetic-like conditions are limited and often confounded by supraphysiological IL-6 concentrations. The purpose of this study is to explore the extent by which an exercise-relevant concentration of IL-6 influences the function of pancreatic β-cells exposed to type 2 diabetic-like conditions. Using insulin-secreting INS-1 832/3 cells as an experimental β-cell model, we show that 1-h IL-6 (10 pg/mL) has no effect on insulin secretion under normal conditions and does not restore the loss of insulin secretion caused by elevated glucose ± palmitate or IL-1β. Moreover, treatment of INS-1 832/3 cells to medium collected from C2C12 myotubes conditioned with electrical pulse stimulation does not alter insulin secretion despite significant increases in IL-6. Since insulin secretory defects caused by diabetic-like conditions are neither improved nor worsened by exposure to physiological IL-6 levels, we conclude that the beneficial effect of exercise on β-cell function is unlikely to be driven by muscle-derived IL-6. View Full-Text
Keywords: pancreatic beta cell dysfunction; insulin secretion; interleukin-6; type 2 diabetes; exercise; skeletal muscle; electrical pulse stimulation; organ crosstalk pancreatic beta cell dysfunction; insulin secretion; interleukin-6; type 2 diabetes; exercise; skeletal muscle; electrical pulse stimulation; organ crosstalk
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MDPI and ACS Style

Barlow, J.; Carter, S.; Solomon, T.P.J. Probing the Effect of Physiological Concentrations of IL-6 on Insulin Secretion by INS-1 832/3 Insulinoma Cells under Diabetic-Like Conditions. Int. J. Mol. Sci. 2018, 19, 1924. https://doi.org/10.3390/ijms19071924

AMA Style

Barlow J, Carter S, Solomon TPJ. Probing the Effect of Physiological Concentrations of IL-6 on Insulin Secretion by INS-1 832/3 Insulinoma Cells under Diabetic-Like Conditions. International Journal of Molecular Sciences. 2018; 19(7):1924. https://doi.org/10.3390/ijms19071924

Chicago/Turabian Style

Barlow, Jonathan; Carter, Steven; Solomon, Thomas P.J. 2018. "Probing the Effect of Physiological Concentrations of IL-6 on Insulin Secretion by INS-1 832/3 Insulinoma Cells under Diabetic-Like Conditions" Int. J. Mol. Sci. 19, no. 7: 1924. https://doi.org/10.3390/ijms19071924

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