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Open AccessArticle

Macrophage Populations in Visceral Adipose Tissue from Pregnant Women: Potential Role of Obesity in Maternal Inflammation

1
Department of Immunobiochemistry, Instituto Nacional de Perinatologia, 11000 Mexico City, Mexico
2
Posgrado en Ciencias Biologicas, Universidad Nacional Autonoma de Mexico, 0451110 Mexico City, Mexico
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Department of Infectology and Immunology, Instituto Nacional de Perinatologia, 11000 Mexico City, Mexico
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Clinical Research Branch, Instituto Nacional de Perinatologia, 11000 Mexico City, Mexico
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Department of Obstetrics, Instituto Nacional de Perinatologia, 11000 Mexico City, Mexico
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Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutricion Salvador Zubiran, 14080 Mexico City, Mexico
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Department of Human Genetics and Genomics, Instituto Nacional de Perinatologia, 11000 Mexico City, Mexico
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Department of Nutrition and Bioprogramming, Instituto Nacional de Perinatologia, 11000 Mexico City, Mexico
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Endocrinology Division, Instituto Nacional de Perinatologia, 11000 Mexico City, Mexico
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Research Division, Instituto Nacional de Perinatologia, 11000 Mexico City, Mexico
*
Author to whom correspondence should be addressed.
E.B.F. is a doctoral student in the Programa de Doctorado en Ciencias Biologicas, Universidad Nacional Autonoma de Mexico (UNAM). This work is from a dissertation to be presented in partial fulfillment of the requirements for the Ph.D. degree.
Int. J. Mol. Sci. 2018, 19(4), 1074; https://doi.org/10.3390/ijms19041074
Received: 15 February 2018 / Revised: 22 March 2018 / Accepted: 26 March 2018 / Published: 4 April 2018
(This article belongs to the Special Issue Macrophages in Inflammation)
Obesity is associated with inflammatory changes and accumulation and phenotype polarization of adipose tissue macrophages (ATMs). Obese pregnant women have alterations in adipose tissue composition, but a detailed description of macrophage population is not available. In this study, we characterized macrophage populations in visceral adipose tissue (VAT) from pregnant women with normal, overweight, and obese pregestational weight. Immunophenotyping of macrophages from VAT biopsies was performed by flow cytometry using CD45 and CD14 as markers of hematopoietic and monocyte linage, respectively, while HLA-DR, CD11c, CD163, and CD206 were used as pro- and anti-inflammatory markers. Adipocyte number and size were evaluated by light microscopy. The results show that pregnant women that were overweight and obese during the pregestational period had adipocyte hypertrophy. Two different macrophage populations in VAT were identified: recruited macrophages (CD45+CD14+), and a novel population lacking CD45, which was considered to be a resident macrophages subset (CD45CD14+). The number of resident HLADRlow/− macrophages showed a negative correlation with body mass index (BMI). Both resident and recruited macrophages from obese women expressed higher CD206 levels. CD11c expression was higher in resident HLA-DR+ macrophages from obese women. A strong correlation between CD206 and CD11c markers and BMI was observed. Our findings show that being overweight and obese in the pregestational period is associated with adipocyte hypertrophy and specific ATMs populations in VAT. View Full-Text
Keywords: pregnancy; obesity; resident macrophage; recruited macrophage; inflammation; visceral adipose tissue pregnancy; obesity; resident macrophage; recruited macrophage; inflammation; visceral adipose tissue
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Bravo-Flores, E.; Mancilla-Herrera, I.; Espino y Sosa, S.; Ortiz-Ramirez, M.; Flores-Rueda, V.; Ibargüengoitia-Ochoa, F.; Ibañez, C.A.; Zambrano, E.; Solis-Paredes, M.; Perichart-Perera, O.; Sanchez-Martinez, M.; Medina-Bastidas, D.; Reyes-Muñoz, E.; Estrada-Gutierrez, G. Macrophage Populations in Visceral Adipose Tissue from Pregnant Women: Potential Role of Obesity in Maternal Inflammation. Int. J. Mol. Sci. 2018, 19, 1074.

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