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Volume 19
Issue 3
10.3390/ijms19030773
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Article

miR-25-3p, Positively Regulated by Transcription Factor AP-2α, Regulates the Metabolism of C2C12 Cells by Targeting Akt1

by
Feng Zhang
1,2,
Kun Chen
2,
Hu Tao
1,
Tingting Kang
2,
Qi Xiong
1,
Qianhui Zeng
2,
Yang Liu
1,
Siwen Jiang
2,* and
Mingxin Chen
1,*
1
Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China
2
Key Laboratory of Swine Genetics and Breeding of the Agricultural Ministry and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(3), 773; https://doi.org/10.3390/ijms19030773
Received: 15 January 2018 / Revised: 4 March 2018 / Accepted: 6 March 2018 / Published: 8 March 2018
(This article belongs to the Special Issue Transcriptional Regulation: Molecules, Involved Mechanisms and Misregulation)
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Abstract

miR-25, a member of the miR-106b-25 cluster, has been reported as playing an important role in many biological processes by numerous studies, while the role of miR-25 in metabolism and its transcriptional regulation mechanism remain unclear. In this study, gain-of-function and loss-of-function assays demonstrated that miR-25-3p positively regulated the metabolism of C2C12 cells by attenuating phosphoinositide 3-kinase (PI3K) gene expression and triglyceride (TG) content, and enhancing the content of adenosine triphosphate (ATP) and reactive oxygen species (ROS). Furthermore, the results from bioinformatics analysis, dual luciferase assay, site-directed mutagenesis, qRT-PCR, and Western blotting demonstrated that miR-25-3p directly targeted the AKT serine/threonine kinase 1 (Akt1) 3′ untranslated region (3′UTR). The core promoter of miR-25-3p was identified, and the transcription factor activator protein-2α (AP-2α) significantly increased the expression of mature miR-25-3p by binding to its core promoter in vivo, as indicated by the chromatin immunoprecipitation (ChIP) assay, and AP-2α binding also downregulated the expression of Akt1. Taken together, our findings suggest that miR-25-3p, positively regulated by the transcription factor AP-2α, enhances C2C12 cell metabolism by targeting the Akt1 gene. View Full-Text
Keywords: mouse; miR-25-3p; Akt1; AP-2α; promoter; cell metabolism mouse; miR-25-3p; Akt1; AP-2α; promoter; cell metabolism
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MDPI and ACS Style

Zhang, F.; Chen, K.; Tao, H.; Kang, T.; Xiong, Q.; Zeng, Q.; Liu, Y.; Jiang, S.; Chen, M. miR-25-3p, Positively Regulated by Transcription Factor AP-2α, Regulates the Metabolism of C2C12 Cells by Targeting Akt1. Int. J. Mol. Sci. 2018, 19, 773. https://doi.org/10.3390/ijms19030773

AMA Style

Zhang F, Chen K, Tao H, Kang T, Xiong Q, Zeng Q, Liu Y, Jiang S, Chen M. miR-25-3p, Positively Regulated by Transcription Factor AP-2α, Regulates the Metabolism of C2C12 Cells by Targeting Akt1. International Journal of Molecular Sciences. 2018; 19(3):773. https://doi.org/10.3390/ijms19030773

Chicago/Turabian Style

Zhang, Feng, Kun Chen, Hu Tao, Tingting Kang, Qi Xiong, Qianhui Zeng, Yang Liu, Siwen Jiang, and Mingxin Chen. 2018. "miR-25-3p, Positively Regulated by Transcription Factor AP-2α, Regulates the Metabolism of C2C12 Cells by Targeting Akt1" International Journal of Molecular Sciences 19, no. 3: 773. https://doi.org/10.3390/ijms19030773

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