Next Article in Journal
A Complex Relationship between Visfatin and Resistin and microRNA: An In Vitro Study on Human Chondrocyte Cultures
Next Article in Special Issue
MLN4924, a Protein Neddylation Inhibitor, Suppresses the Growth of Human Chondrosarcoma through Inhibiting Cell Proliferation and Inducing Endoplasmic Reticulum Stress-Related Apoptosis
Previous Article in Journal
Genetic Mapping of Loci for Resistance to Stem Rust in a Tetraploid Wheat Collection
Previous Article in Special Issue
Low HIF-1α and low EGFR mRNA Expression Significantly Associate with Poor Survival in Soft Tissue Sarcoma Patients; the Proteins React Differently
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2018, 19(12), 3908; https://doi.org/10.3390/ijms19123908

Defining a Characteristic Gene Expression Set Responsible for Cancer Stem Cell-Like Features in a Sub-Population of Ewing Sarcoma Cells CADO-ES1

1
Department of Pediatric Hematology and Oncology, University Hospital Münster, 48149 Münster, Germany
2
Institute of Bioinformatics, Faculty of Medicine, University of Münster, 48149 Münster, Germany
3
Institute of Human Genetics, Faculty of Medicine, University of Münster, 48149 Münster, Germany
4
University Hospital Essen, Pediatrics III, Hematology and Oncology, West German Cancer Centre, 45147 Essen, Germany
*
Author to whom correspondence should be addressed.
Authors contribute equally.
Received: 22 October 2018 / Revised: 26 November 2018 / Accepted: 4 December 2018 / Published: 6 December 2018
(This article belongs to the Special Issue Current Advances in Soft Tissue and Bone Sarcoma)
Full-Text   |   PDF [2767 KB, uploaded 12 December 2018]   |  
  |   Review Reports

Abstract

One of the still open questions in Ewing sarcoma, a rare bone tumor with weak therapeutic options, is to identify the tumor-driving cell (sub) population and to understand the specifics in the biological network of these cells. This basic scientific insight might foster the development of more specific therapeutic target patterns. The experimental approach is based on a side population (SP) of Ewing cells, based on the model cell line CADO-ES1. The SP is established by flow cytometry and defined by the idea that tumor stem-like cells can be identified by the time-course in clearing a given artificial dye. The SP was characterized by a higher colony forming activity, by a higher differentiation potential, by higher resistance to cytotoxic drugs, and by morphology. Several SP and non-SP cell fractions and bone marrow-derived mesenchymal stem cell reference were analyzed by short read sequencing of the full transcriptome. The double-differential analysis leads to an altered expression structure of SP cells centered around the AP-1 and APC/c complex. The SP cells share only a limited proportion of the full mesenchymal stem cell stemness set of genes. This is in line with the expectation that tumor stem-like cells share only a limited subset of stemness features which are relevant for tumor survival. View Full-Text
Keywords: Ewing sarcoma; side population; tumor driver cells; cancer stem cells; mesenchymal stem cells; gene expression; AP-1; APC/c Ewing sarcoma; side population; tumor driver cells; cancer stem cells; mesenchymal stem cells; gene expression; AP-1; APC/c
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary materials

SciFeed

Share & Cite This Article

MDPI and ACS Style

Hotfilder, M.; Mallela, N.; Seggewiß, J.; Dirksen, U.; Korsching, E. Defining a Characteristic Gene Expression Set Responsible for Cancer Stem Cell-Like Features in a Sub-Population of Ewing Sarcoma Cells CADO-ES1. Int. J. Mol. Sci. 2018, 19, 3908.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top