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Open AccessCommunication

Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line

1
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Lomonosov Moscow State University, 119991 Moscow, Russia
2
Laboratory of Molecular Endocrinology, Russian Cardiology Research Center, 121552 Moscow, Russia
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(12), 3712; https://doi.org/10.3390/ijms19123712
Received: 23 October 2018 / Revised: 15 November 2018 / Accepted: 20 November 2018 / Published: 22 November 2018
(This article belongs to the Special Issue Single Cell Technology)
Primary adipose tissue-derived multipotent stem/stromal cells (adMSCs) demonstrate unusual signaling regulatory mechanisms, i.e., increased of sensitivity to catecholamines in response to noradrenaline. This phenomenon is called “heterologous sensitization”, and was previously found only in embryonic cells. Since further elucidation of the molecular mechanisms that are responsible for such sensitization in primary adMSCs was difficult due to the high heterogeneity in adrenergic receptor expression, we employed immortalized adipose-derived mesenchymal stem cell lines (hTERT-MSCs). Using flow cytometry and immunofluorescence microscopy, we demonstrated that the proportion of cells expressing adrenergic receptor isoforms does not differ significantly in hTERT-MSCs cells compared to the primary adMSCs culture. However, using analysis of Ca2+-mobilization in single cells, we found that these cells did not demonstrate the sensitization seen in primary adMSCs. Consistently, these cells did not activate cAMP synthesis in response to noradrenaline. These data indicate that immortalized adipose-derived mesenchymal stem cell lines demonstrated impaired ability to respond to noradrenaline compared to primary adMSCs. These data draw attention to the usage of immortalized cells for MSCs-based regenerative medicine, especially in the field of pharmacology. View Full-Text
Keywords: mesenchymal stem/stromal cells; noradrenaline; norepinephrine; intracellular signaling; membrane receptors; adrenergic receptors; calcium imaging; ASC52telo; hTERT mesenchymal stem/stromal cells; noradrenaline; norepinephrine; intracellular signaling; membrane receptors; adrenergic receptors; calcium imaging; ASC52telo; hTERT
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Tyurin-Kuzmin, P.A.; Chechekhin, V.I.; Ivanova, A.M.; Dyikanov, D.T.; Sysoeva, V.Y.; Kalinina, N.I.; Tkachuk, V.A. Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line. Int. J. Mol. Sci. 2018, 19, 3712.

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