Next Article in Journal
Lowering Low-Density Lipoprotein Particles in Plasma Using Dextran Sulphate Co-Precipitates Procoagulant Extracellular Vesicles
Next Article in Special Issue
Understanding the Role of Intrinsic Disorder of Viral Proteins in the Oncogenicity of Different Types of HPV
Previous Article in Journal
MicroRNA-27b Depletion Enhances Endotrophic and Intravascular Lipid Accumulation and Induces Adipocyte Hyperplasia in Zebrafish
Previous Article in Special Issue
Functional Analysis of Human Hub Proteins and Their Interactors Involved in the Intrinsic Disorder-Enriched Interactions
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2018, 19(1), 91;

InSiDDe: A Server for Designing Artificial Disordered Proteins

Aix-Marseille University, CNRS, Architecture et Fonction des Macromolécules Biologiques (AFMB), UMR 7257 Marseille, France
Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Istituto di Biologia e Patologia Molecolari del CNR, Dipartimento di Scienze Biochimiche “A. Rossi Fanelli”, Sapienza Università di Roma, 00185 Rome, Italy
Authors to whom correspondence should be addressed.
Received: 24 November 2017 / Revised: 21 December 2017 / Accepted: 22 December 2017 / Published: 29 December 2017
Full-Text   |   PDF [4181 KB, uploaded 29 December 2017]   |  


InSiDDe (In Silico Disorder Design) is a program for the in silico design of intrinsically disordered proteins of desired length and disorder probability. The latter is assessed using IUPred and spans values ranging from 0.55 to 0.95 with 0.05 increments. One to ten artificial sequences per query, each made of 50 to 200 residues, can be generated by InSiDDe. We describe the rationale used to set up InSiDDe and show that an artificial sequence of 100 residues with an IUPred score of 0.6 designed by InSiDDe could be recombinantly expressed in E. coli at high levels without degradation when fused to a natural molecular recognition element (MoRE). In addition, the artificial fusion protein exhibited the expected behavior in terms of binding modulation of the specific partner recognized by the MoRE. To the best of our knowledge, InSiDDe is the first publicly available software for the design of intrinsically disordered protein (IDP) sequences. InSiDDE is publicly available online. View Full-Text
Keywords: intrinsically disordered proteins; artificial protein; IUPred; recombinant protein; Python3; paramyxovirus; NTAIL; XD; E. coli intrinsically disordered proteins; artificial protein; IUPred; recombinant protein; Python3; paramyxovirus; NTAIL; XD; E. coli

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Schramm, A.; Lieutaud, P.; Gianni, S.; Longhi, S.; Bignon, C. InSiDDe: A Server for Designing Artificial Disordered Proteins. Int. J. Mol. Sci. 2018, 19, 91.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top