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Int. J. Mol. Sci. 2017, 18(12), 2530; https://doi.org/10.3390/ijms18122530

BCL11A mRNA Targeting by miR-210: A Possible Network Regulating γ-Globin Gene Expression

1
Department of Life Sciences and Biotechnology, Ferrara University, 44121 Ferrara, Italy
2
Laboratory for the Development of Pharmacological and Pharmacogenomic Therapy of Thalassaemia, Biotechnology Center, Ferrara University, 44121 Ferrara, Italy
*
Authors to whom correspondence should be addressed.
Received: 23 October 2017 / Revised: 16 November 2017 / Accepted: 22 November 2017 / Published: 26 November 2017
(This article belongs to the Collection Regulation by Non-Coding RNAs)
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Abstract

The involvement of microRNAs in the control of repressors of human γ-globin gene transcription has been firmly demonstrated, as described for the miR-486-3p mediated down-regulation of BCL11A. On the other hand, we have reported that miR-210 is involved in erythroid differentiation and, possibly, in γ-globin gene up-regulation. In the present study, we have identified the coding sequence of BCL11A as a possible target of miR-210. The following results sustain this hypothesis: (a) interactions between miR-210 and the miR-210 BCL11A site were demonstrated by SPR-based biomolecular interaction analysis (BIA); (b) the miR-210 site of BCL11A is conserved through molecular evolution; (c) forced expression of miR-210 leads to decrease of BCL11A-XL and increase of γ-globin mRNA content in erythroid cells, including erythroid precursors isolated from β-thalassemia patients. Our study suggests that the coding mRNA sequence of BCL11A can be targeted by miR-210. In addition to the theoretical point of view, these data are of interest from the applied point of view, supporting a novel strategy to inhibit BCL11A by mimicking miR-210 functions, accordingly with the concept supported by several papers and patent applications that inhibition of BCL11A is an efficient strategy for fetal hemoglobin induction in the treatment of β-thalassemia. View Full-Text
Keywords: microRNAs; miR-210; BCL11A; hemoglobin; erythroid differentiation; β-thalassemia; γ-globin microRNAs; miR-210; BCL11A; hemoglobin; erythroid differentiation; β-thalassemia; γ-globin
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Gasparello, J.; Fabbri, E.; Bianchi, N.; Breveglieri, G.; Zuccato, C.; Borgatti, M.; Gambari, R.; Finotti, A. BCL11A mRNA Targeting by miR-210: A Possible Network Regulating γ-Globin Gene Expression. Int. J. Mol. Sci. 2017, 18, 2530.

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