Efficient Synthesis of Peptide and Protein Functionalized Pyrrole-Imidazole Polyamides Using Native Chemical Ligation
Abstract
:1. Introduction
2. Results
2.1. Synthesis of Cysteine-Functionalized Pyrrole-Imidazole (Py–Im) Polyamide
2.2. Native Chemical Ligation of Thioester Peptides and Proteins to Cys-Py–Im-Polyamides
2.3. Effect of Conjugation on DNA Binding Properties
3. Discussion and Conclusion
4. Experimental Section
4.1. Fmoc-Mediated Solid Phase Synthesis of the Non- and Tert-BuSH Protected Cysteine Py–Im Polyamide
4.2. Fmoc-Mediated Solid Phase Peptide Synthesis of Nbz-Peptide
4.3. Protein Expression and Purification
4.4. Native Chemical Ligation Reactions
4.5. Surface Plasmon Resonance
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Janssen, B.M.G.; Van Ommeren, S.P.F.I.; Merkx, M. Efficient Synthesis of Peptide and Protein Functionalized Pyrrole-Imidazole Polyamides Using Native Chemical Ligation. Int. J. Mol. Sci. 2015, 16, 12631-12647. https://doi.org/10.3390/ijms160612631
Janssen BMG, Van Ommeren SPFI, Merkx M. Efficient Synthesis of Peptide and Protein Functionalized Pyrrole-Imidazole Polyamides Using Native Chemical Ligation. International Journal of Molecular Sciences. 2015; 16(6):12631-12647. https://doi.org/10.3390/ijms160612631
Chicago/Turabian StyleJanssen, Brian M. G., Sven P. F. I. Van Ommeren, and Maarten Merkx. 2015. "Efficient Synthesis of Peptide and Protein Functionalized Pyrrole-Imidazole Polyamides Using Native Chemical Ligation" International Journal of Molecular Sciences 16, no. 6: 12631-12647. https://doi.org/10.3390/ijms160612631