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Open AccessArticle

Dietary Compound Kaempferol Inhibits Airway Thickening Induced by Allergic Reaction in a Bovine Serum Albumin-Induced Model of Asthma

Department of Food and Nutrition, Hallym University, Chuncheon 200-702, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Paula Andrade
Int. J. Mol. Sci. 2015, 16(12), 29980-29995; https://doi.org/10.3390/ijms161226218
Received: 21 October 2015 / Revised: 26 November 2015 / Accepted: 8 December 2015 / Published: 16 December 2015
(This article belongs to the Special Issue Phenolics and Polyphenolics 2015)
Asthma is characterized by aberrant airways including epithelial thickening, goblet cell hyperplasia, and smooth muscle hypertrophy within the airway wall. The current study examined whether kaempferol inhibited mast cell degranulation and prostaglandin (PG) release leading to the development of aberrant airways, using an in vitro model of dinitrophenylated bovine serum albumin (DNP-BSA)-sensitized rat basophilic leukemia (RBL-2H3) mast cells and an in vivo model of BSA-challenged asthmatic mice. Nontoxic kaempferol at 10–20 μM suppressed β-hexosaminidase release and cyclooxygenase 2 (COX2)-mediated production of prostaglandin D2 (PGD2) and prostaglandin F2α (PGF2α) in sensitized mast cells. Oral administration of ≤20 mg/kg kaempferol blocked bovine serum albumin (BSA) inhalation-induced epithelial cell excrescence and smooth muscle hypertrophy by attenuating the induction of COX2 and the formation of PGD2 and PGF2α, together with reducing the anti-α-smooth muscle actin (α-SMA) expression in mouse airways. Kaempferol deterred the antigen-induced mast cell activation of cytosolic phospholipase A2 (cPLA2) responsive to protein kinase Cμ (PKCμ) and extracellular signal-regulated kinase (ERK). Furthermore, the antigen-challenged activation of Syk-phospholipase Cγ (PLCγ) pathway was dampened in kaempferol-supplemented mast cells. These results demonstrated that kaempferol inhibited airway wall thickening through disturbing Syk-PLCγ signaling and PKCμ-ERK-cPLA2-COX2 signaling in antigen-exposed mast cells. Thus, kaempferol may be a potent anti-allergic compound targeting allergic asthma typical of airway hyperplasia and hypertrophy. View Full-Text
Keywords: airway thickening; asthma; bronchoconstriction; cyclooxygenase 2; kaempferol airway thickening; asthma; bronchoconstriction; cyclooxygenase 2; kaempferol
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MDPI and ACS Style

Shin, D.; Park, S.-H.; Choi, Y.-J.; Kim, Y.-H.; Antika, L.D.; Habibah, N.U.; Kang, M.-K.; Kang, Y.-H. Dietary Compound Kaempferol Inhibits Airway Thickening Induced by Allergic Reaction in a Bovine Serum Albumin-Induced Model of Asthma. Int. J. Mol. Sci. 2015, 16, 29980-29995. https://doi.org/10.3390/ijms161226218

AMA Style

Shin D, Park S-H, Choi Y-J, Kim Y-H, Antika LD, Habibah NU, Kang M-K, Kang Y-H. Dietary Compound Kaempferol Inhibits Airway Thickening Induced by Allergic Reaction in a Bovine Serum Albumin-Induced Model of Asthma. International Journal of Molecular Sciences. 2015; 16(12):29980-29995. https://doi.org/10.3390/ijms161226218

Chicago/Turabian Style

Shin, Daekeun; Park, Sin-Hye; Choi, Yean-Jung; Kim, Yun-Ho; Antika, Lucia D.; Habibah, Nurina U.; Kang, Min-Kyung; Kang, Young-Hee. 2015. "Dietary Compound Kaempferol Inhibits Airway Thickening Induced by Allergic Reaction in a Bovine Serum Albumin-Induced Model of Asthma" Int. J. Mol. Sci. 16, no. 12: 29980-29995. https://doi.org/10.3390/ijms161226218

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