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Int. J. Mol. Sci. 2014, 15(8), 14372-14385;

Beclin 1 Expression is Closely Linked to Colorectal Carcinogenesis and Distant Metastasis of Colorectal Carcinoma

Laboratory Animal Center, China Medical University, Shenyang 110001, China
Department of Biochemistry and Molecular Biology, College of Basic Medicine, China Medical University, Shenyang 110001, China
Department of Surgical Oncology, the First Affiliated Hospital of China Medical University, Shenyang 110001, China
Clinical Research Institute, Kanagawa Cancer Center, Yokohama 241-0815, Japan
Author to whom correspondence should be addressed.
Received: 14 June 2014 / Revised: 29 July 2014 / Accepted: 11 August 2014 / Published: 18 August 2014
(This article belongs to the Section Biochemistry)
PDF [2754 KB, uploaded 18 August 2014]


Beclin 1 participates in development, autophagy, differentiation, anti- apoptosis, neurodegeneration, tumorigenesis and cancer progression. The roles of Beclin 1 in colorectal carcinogenesis and its subsequent progression are still unclear. Here, the mRNA and protein expression of Beclin 1 were determined in colorectal carcinoma and matched mucosa by Reverse transcriptase-polymerase chain reaction and Western blot. Immunohistochemistry and in situ hybridization (ISH) were performed on tissue microarryer with colorectal carcinoma, adenoma and mucosa. The expression of Beclin 1 mRNA and protein was found to be higher in colorectal carcinoma than matched mucosa by real-time PCR and Western blot (p < 0.05). According to the ISH data, Beclin 1 expression was lower in colorectal non-neoplastic mucosa (NNM) than adenoma and carcinoma (p < 0.05). Immunohistochemically, primary carcinoma showed stronger Beclin 1 expression than NNM and metastatic carcinoma in the liver (p < 0.05). Beclin 1 protein expression was negatively related to liver and distant metastasis (p < 0.05), but not correlated with age, sex, depth of invasion, lymphatic or venous invasion, lymph node metastasis, tumor-node-metastasis (TNM) staging, differentiation or serum carcinoembryonic antigen (CEA) concentration (p > 0.05). Survival analysis indicated that Beclin 1 expression was not linked to favorable prognosis of the patients with colorectal carcinoma (p > 0.05). Cox’s model indicated that depth of invasion and distant metastasis were independent prognostic factors for colorectal carcinomas (p < 0.05). It was suggested that Beclin 1 expression is closely linked to colorectal carcinogenesis and distant metastasis of colorectal carcinoma. View Full-Text
Keywords: colorectal carcinoma; Beclin 1; tumorigenesis; pathogenesis; prognosis colorectal carcinoma; Beclin 1; tumorigenesis; pathogenesis; prognosis

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Zhang, M.-Y.; Gou, W.-F.; Zhao, S.; Mao, X.-Y.; Zheng, Z.-H.; Takano, Y.; Zheng, H.-C. Beclin 1 Expression is Closely Linked to Colorectal Carcinogenesis and Distant Metastasis of Colorectal Carcinoma. Int. J. Mol. Sci. 2014, 15, 14372-14385.

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