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Open AccessArticle

A Novel Human TGF-β1 Fusion Protein in Combination with rhBMP-2 Increases Chondro-Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

1
Laboratory of Bioengineering and Tissue Regeneration (LABRET), Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, Universidad de Málaga, Campus de Teatinos, Málaga 29071, Spain
2
Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
3
BIONAND, Centro Andaluz de Nanomedicina y Biotecnología (Junta de Andalucía), Universidad de Málaga, Málaga 29590, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(7), 11255-11274; https://doi.org/10.3390/ijms150711255
Received: 1 May 2014 / Revised: 3 June 2014 / Accepted: 10 June 2014 / Published: 25 June 2014
(This article belongs to the Special Issue The Chondrocyte Phenotype in Cartilage Biology)
Transforming growth factor-beta (TGF-β) is involved in processes related to the differentiation and maturation of osteoprogenitor cells into osteoblasts. Rat bone marrow (BM) cells were cultured in a collagen-gel containing 0.5% fetal bovine serum (FBS) for 10 days in the presence of rhTGF (recombinant human TGF)-β1-F2, a fusion protein engineered to include a high-affinity collagen-binding decapeptide derived from von Willebrand factor. Subsequently, cells were moderately expanded in medium with 10% FBS for 4 days and treated with a short pulse of rhBMP (recombinant human bone morphogenetic protein)-2 for 4 h. During the last 2 days, dexamethasone and β-glycerophosphate were added to potentiate osteoinduction. Concomitant with an up-regulation of cell proliferation, DNA synthesis levels were determined. Polymerase chain reaction was performed to reveal the possible stemness of these cells. Osteogenic differentiation was evaluated in terms of alkaline phosphatase activity and mineralized matrix formation as well as by mRNA expression of osteogenic marker genes. Moreover, cells were placed inside diffusion chambers and implanted subcutaneously into the backs of adult rats for 4 weeks. Histological study provided evidence of cartilage and bone-like tissue formation. This experimental procedure is capable of selecting cell populations from BM that, in the presence of rhTGF-β1-F2 and rhBMP-2, achieve skeletogenic potential in vitro and in vivo. View Full-Text
Keywords: stem/progenitor cell; three-dimensional (3D) culture; transforming growth factor-beta1 (TGF-β1); bone morphogenetic protein-2 (BMP-2); osteogenesis; chondrogenesis stem/progenitor cell; three-dimensional (3D) culture; transforming growth factor-beta1 (TGF-β1); bone morphogenetic protein-2 (BMP-2); osteogenesis; chondrogenesis
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Claros, S.; Rico-Llanos, G.A.; Becerra, J.; Andrades, J.A. A Novel Human TGF-β1 Fusion Protein in Combination with rhBMP-2 Increases Chondro-Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells. Int. J. Mol. Sci. 2014, 15, 11255-11274.

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