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Open AccessArticle

Diagnosis of Desmoplastic Reaction by Immunohistochemical Analysis, in Biopsy Specimens of Early Colorectal Carcinomas, Is Efficacious in Estimating the Depth of Invasion

1
Department of Surgical and Molecular Pathology, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Shimotsuga, Tochigi 321-0293, Japan
2
Department of Gastroenterology, Shizuoka City Shizuoka Hospital, 10-93 Otemachi, Aoi-ku, Shizuoka City, Shizuoka 420-8630, Japan
3
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Toho University Omori Medical Center, 6-11-1 Omorinishi, Ota-ku, Tokyo 143-8541, Japan
4
First Department of Surgery, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Shimotsuga, Tochigi 321-0293, Japan
5
Department of Diagnostic Pathology, Graduate School of Medicine and Pharmaceutical Science, University of Toyama, 2630 Sugitani, Toyama City, Toyama 930-0194, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2013, 14(7), 13129-13136; https://doi.org/10.3390/ijms140713129
Received: 3 May 2013 / Revised: 3 June 2013 / Accepted: 7 June 2013 / Published: 25 June 2013
(This article belongs to the Special Issue Pathogenesis and Prevention of Colorectal Cancer)
The aim of our study was to evaluate the diagnosis of desmoplastic reaction (DR) by immunostaining for α-smooth muscle actin (αSMA) and desmin, for predicting the depth of submucosal invasion in biopsy specimens of early colorectal carcinomas (CRCs). Thirty-eight cases of non-pedunculated early CRCs were included in this study. Positive for DR was defined as αSMA-positive and desmin-negative stroma in the CRC. The depth of submucosal invasion was measured in endoscopically or surgically resected specimens and the lesions were subsequently divided into two groups: Group A (carcinoma in situ/intramucosal carcinoma and submucosal invasive carcinoma with a depth <1000 μm) and Group B (submucosal invasion with a depth ≥1000 μm). Twenty-one cases were DR-positive and 17 were DR-negative. No statistical significance was found between the DR with regard to tumor size, location and histological type. All DR-positive cases belonged to Group B whereas 14 (82.4%) DR-negative lesions belonged to Group A (p < 0.001). The sensitivity, specificity, positive and negative predictive values and accuracy of DR positivity for diagnosis of Group B were 87.5%, 100%, 100%, 82.4% and 92.1%, respectively. Conclusively, detection of DR in biopsy specimens with ancillary immunohistochemistry (αSMA/desmin) would help in preoperative diagnosis for the depth of submucosal invasion of early CRC. View Full-Text
Keywords: immunohistochemistry; biopsy; desmoplastic reaction; early colorectal carcinoma; submucosal invasion immunohistochemistry; biopsy; desmoplastic reaction; early colorectal carcinoma; submucosal invasion
MDPI and ACS Style

Ohno, K.; Fujimori, T.; Okamoto, Y.; Ichikawa, K.; Yamaguchi, T.; Imura, J.; Tomita, S.; Mitomi, H. Diagnosis of Desmoplastic Reaction by Immunohistochemical Analysis, in Biopsy Specimens of Early Colorectal Carcinomas, Is Efficacious in Estimating the Depth of Invasion. Int. J. Mol. Sci. 2013, 14, 13129-13136. https://doi.org/10.3390/ijms140713129

AMA Style

Ohno K, Fujimori T, Okamoto Y, Ichikawa K, Yamaguchi T, Imura J, Tomita S, Mitomi H. Diagnosis of Desmoplastic Reaction by Immunohistochemical Analysis, in Biopsy Specimens of Early Colorectal Carcinomas, Is Efficacious in Estimating the Depth of Invasion. International Journal of Molecular Sciences. 2013; 14(7):13129-13136. https://doi.org/10.3390/ijms140713129

Chicago/Turabian Style

Ohno, Kazuya; Fujimori, Takahiro; Okamoto, Yosuke; Ichikawa, Kazuhito; Yamaguchi, Takeshi; Imura, Johji; Tomita, Shigeki; Mitomi, Hiroyuki. 2013. "Diagnosis of Desmoplastic Reaction by Immunohistochemical Analysis, in Biopsy Specimens of Early Colorectal Carcinomas, Is Efficacious in Estimating the Depth of Invasion" Int. J. Mol. Sci. 14, no. 7: 13129-13136. https://doi.org/10.3390/ijms140713129

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