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Article

Frataxin Deficiency Leads to Reduced Expression and Impaired Translocation of NF-E2-Related Factor (Nrf2) in Cultured Motor Neurons

1
Confocal Microscopy Core Facility, Research Laboratories, Bambino Gesù Children's Hospital, IRCCS, Rome 00165, Italy
2
Unit for Neuromuscular and Neurodegenerative Diseases, Bambino Gesù Children’s Hospital, IRCCS, Piazza S’Onofrio, 4, Rome 00165, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to the study.
Int. J. Mol. Sci. 2013, 14(4), 7853-7865; https://doi.org/10.3390/ijms14047853
Received: 1 March 2013 / Revised: 29 March 2013 / Accepted: 2 April 2013 / Published: 10 April 2013
(This article belongs to the Special Issue Redox Signaling in Biology and Patho-Biology)
Oxidative stress has been implicated in the pathogenesis of Friedreich’s Ataxia (FRDA), a neurodegenerative disease caused by the decreased expression of frataxin, a mitochondrial protein responsible of iron homeostasis. Under conditions of oxidative stress, the activation of the transcription factor NF-E2-related factor (Nrf2) triggers the antioxidant cellular response by inducing antioxidant response element (ARE) driven genes. Increasing evidence supports a role for the Nrf2-ARE pathway in neurodegenerative diseases. In this study, we analyzed the expression and the distribution of Nrf2 in silenced neurons for frataxin gene. Decreased Nrf2 mRNA content and a defective activation after treatment with pro-oxidants have been evidenced in frataxin-silenced neurons by RT-PCR and confocal microscopy. The loss of Nrf2 in FRDA may greatly enhance the cellular susceptibility to oxidative stress and make FRDA neurons more vulnerable to injury. Our findings may help to focus on this promising target, especially in its emerging role in the neuroprotective response. View Full-Text
Keywords: FRDA; frataxin; Nrf2; GSSG; oxidative stress; NSC34 neurons FRDA; frataxin; Nrf2; GSSG; oxidative stress; NSC34 neurons
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MDPI and ACS Style

D'Oria, V.; Petrini, S.; Travaglini, L.; Priori, C.; Piermarini, E.; Petrillo, S.; Carletti, B.; Bertini, E.; Piemonte, F. Frataxin Deficiency Leads to Reduced Expression and Impaired Translocation of NF-E2-Related Factor (Nrf2) in Cultured Motor Neurons. Int. J. Mol. Sci. 2013, 14, 7853-7865. https://doi.org/10.3390/ijms14047853

AMA Style

D'Oria V, Petrini S, Travaglini L, Priori C, Piermarini E, Petrillo S, Carletti B, Bertini E, Piemonte F. Frataxin Deficiency Leads to Reduced Expression and Impaired Translocation of NF-E2-Related Factor (Nrf2) in Cultured Motor Neurons. International Journal of Molecular Sciences. 2013; 14(4):7853-7865. https://doi.org/10.3390/ijms14047853

Chicago/Turabian Style

D'Oria, Valentina; Petrini, Stefania; Travaglini, Lorena; Priori, Chiara; Piermarini, Emanuela; Petrillo, Sara; Carletti, Barbara; Bertini, Enrico; Piemonte, Fiorella. 2013. "Frataxin Deficiency Leads to Reduced Expression and Impaired Translocation of NF-E2-Related Factor (Nrf2) in Cultured Motor Neurons" Int. J. Mol. Sci. 14, no. 4: 7853-7865. https://doi.org/10.3390/ijms14047853

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