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Int. J. Mol. Sci. 2013, 14(4), 7571-7582;

Quantification of Maternal Serum Cell-Free Fetal DNA in Early-Onset Preeclampsia

Department of Obstetrics and Gynecology, Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, China
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
Department of Microbiology and Immunology, Medical School, Southeast University, Nanjing 210009, China
Authors to whom correspondence should be addressed.
Received: 18 February 2013 / Revised: 12 March 2013 / Accepted: 25 March 2013 / Published: 8 April 2013
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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The aim of this study was to determine whether the increased serum cell-free fetal DNA (cffDNA) level of gravidas developed into early-onset preeclampsia (EOPE) subsequently in the early second trimesters is related to prenatal screening markers. Serum was collected from 1011 gravidas. The level of cffDNA and prenatal screening markers were analyzed in 20 cases with EOPE and 20 controls. All fetuses were male. The maternal serum cffDNA level was assessed by amplification of the Y chromosome specific gene. Correlations between the variables were examined. (Logged) cffDNA in EOPE (median, 3.08; interquartile range, 2.93–3.68) was higher than controls (median, 1.79; interquartile range, 1.46–2.53). The increased level of (logged) cffDNA was correlated significantly with the increased human chorionic gonadotropin (HCG) level (r = 0.628, p < 0.001). Significant reciprocal correlations between cffDNA and babies’ birth weight as well as gestation weeks at delivery were noted (r = −0.516, p = 0.001; r = −0.623, p < 0.001, respectively). The sensitivity and specificity of cffDNA to discriminate between the EOPE cases and the controls were 90% and 85%, respectively. CffDNA is a potential marker for EOPE, which had a significant reciprocal correlation with babies’ birth weight and gestation weeks at delivery. Moreover, it may help in indicating the underlying hypoxic condition in the placenta. View Full-Text
Keywords: preeclampsia; cell-free fetal DNA; HCG preeclampsia; cell-free fetal DNA; HCG
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Yu, H.; Shen, Y.; Ge, Q.; He, Y.; Qiao, D.; Ren, M.; Zhang, J. Quantification of Maternal Serum Cell-Free Fetal DNA in Early-Onset Preeclampsia. Int. J. Mol. Sci. 2013, 14, 7571-7582.

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