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Volume 13
Issue 5
10.3390/ijms13056266
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Article

Low Diversity Bacterial Community and the Trapping Activity of Metabolites from Cultivable Bacteria Species in the Female Reproductive System of the Oriental Fruit Fly, Bactrocera dorsalis Hendel (Diptera: Tephritidae)

by
Zhanghong Shi
†,
Lili Wang
† and
Hongyu Zhang
*
State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Pests, Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2012, 13(5), 6266-6278; https://doi.org/10.3390/ijms13056266
Received: 28 March 2012 / Revised: 4 May 2012 / Accepted: 4 May 2012 / Published: 21 May 2012
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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Abstract

Our goal was to identify the bacteria inhabiting the reproductive system of the female oriental fruit fly, Bactrocera dorsalis (Hendel), and evaluate the chemotaxis of B. dorsalis to the metabolites produced by the bacteria. Based on 16S rRNA-based polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), 18 operational taxonomic units (OTUs) were assigned to the five bacterial classes Betaproteobacteria, Alphaproteobacteria, Gammaproteobacteria, Bacilli and Actinobacteria. Nine OTUs were assigned to Gammaproteobacteria, which was the most highly represented class. Enterobacteriaceae constituted the dominant family, and within this family, three genera and five species were identified, including Enterobacter sakazakii, Klebsiella oxytoca, Klebsiella pneumoniae, Raoultella terrigena and Enterobacter amnigenus. In this set, the first two species were the dominant components, and the latter three species were the minor ones. Finally, we found that the metabolites produced by R. terrigena, K. oxytoca and K. pneumoniae were attractive to the B. dorsalis adults, and in field studies, B. dorsalis adults were most attracted to K. oxytoca. Collectively, our results suggest that the female reproductive system plays an important role in the transfer of enterobacteria from the gut to fruit. Our data may prompt the development of a female-targeted population control strategy for this fly. View Full-Text
Keywords: bacteria; symbiosis; microbiota; Enterobacteriaceae; Klebsiella oxytoca bacteria; symbiosis; microbiota; Enterobacteriaceae; Klebsiella oxytoca
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This is an open access article distributed under the Creative Commons Attribution License
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MDPI and ACS Style

Shi, Z.; Wang, L.; Zhang, H. Low Diversity Bacterial Community and the Trapping Activity of Metabolites from Cultivable Bacteria Species in the Female Reproductive System of the Oriental Fruit Fly, Bactrocera dorsalis Hendel (Diptera: Tephritidae). Int. J. Mol. Sci. 2012, 13, 6266-6278. https://doi.org/10.3390/ijms13056266

AMA Style

Shi Z, Wang L, Zhang H. Low Diversity Bacterial Community and the Trapping Activity of Metabolites from Cultivable Bacteria Species in the Female Reproductive System of the Oriental Fruit Fly, Bactrocera dorsalis Hendel (Diptera: Tephritidae). International Journal of Molecular Sciences. 2012; 13(5):6266-6278. https://doi.org/10.3390/ijms13056266

Chicago/Turabian Style

Shi, Zhanghong, Lili Wang, and Hongyu Zhang. 2012. "Low Diversity Bacterial Community and the Trapping Activity of Metabolites from Cultivable Bacteria Species in the Female Reproductive System of the Oriental Fruit Fly, Bactrocera dorsalis Hendel (Diptera: Tephritidae)" International Journal of Molecular Sciences 13, no. 5: 6266-6278. https://doi.org/10.3390/ijms13056266

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