Chromatographic Method for Monitoring of Pesticide Residues and Risk Assessment for Herbal Decoctions Used in Traditional Korean Medicine Clinics
Abstract
:1. Introduction
2. Results
2.1. Test Analysis Method Verification Result
2.1.1. Specificity
2.1.2. Linearity
2.1.3. Accuracy
2.1.4. Limit of Detection and Quantification
2.2. Pesticide Residues in Analyzed Samples
3. Discussion
4. Materials and Methods
4.1. Sample Collection
4.2. Standards and Reagents
4.3. Pretreatment of Samples
- (1)
- Precisely add 10 mL of decoction to a 50 mL centrifuge tube along with 10 mL of acetonitrile containing the internal standard (0.1 mg/kg triphenylphosphate), shake it, and extract it for 1 to 2 min.
- (2)
- Add 4 g of MgSO4, 1 g of NaCl, 1 g of trisodium citrate dihydrate, and 0.5 g of disodium hydrogen citrate sesquihydrate to the centrifuge tube of (1) and shake it for 1 min. After centrifugation (3000 rpm/min, 5 min) to separate the acetonitrile layer and the water layer, mix the acetonitrile layer with the buffer solution, filter it through a PTFE filter (0.2 μm), and analyze it by LC-MS/MS.
- (3)
- In the case of GC-MS/MS, add 1 mL of acetonitrile extract from (2) to a powdered solid phase extraction tube containing 150 mg of MgSO4 and 25 mg of PSA, shake it for 1 min, and centrifuge it (10,000 rpm/min, 2 min). Then, analyze it by GC-MS/MS.
Analysis of Instrument and Instrument Conditions
4.4. Validation of the Test Method
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Kang, S.-M.; Won, J.-H.; Han, J.-E.; Kim, J.-H.; Kim, K.-H.; Jeong, H.-I.; Sung, S.-H. Chromatographic Method for Monitoring of Pesticide Residues and Risk Assessment for Herbal Decoctions Used in Traditional Korean Medicine Clinics. Molecules 2023, 28, 3343. https://doi.org/10.3390/molecules28083343
Kang S-M, Won J-H, Han J-E, Kim J-H, Kim K-H, Jeong H-I, Sung S-H. Chromatographic Method for Monitoring of Pesticide Residues and Risk Assessment for Herbal Decoctions Used in Traditional Korean Medicine Clinics. Molecules. 2023; 28(8):3343. https://doi.org/10.3390/molecules28083343
Chicago/Turabian StyleKang, Se-Mi, Jae-Hee Won, Ji-Eun Han, Jong-Hyun Kim, Kyeong-Han Kim, Hye-In Jeong, and Soo-Hyun Sung. 2023. "Chromatographic Method for Monitoring of Pesticide Residues and Risk Assessment for Herbal Decoctions Used in Traditional Korean Medicine Clinics" Molecules 28, no. 8: 3343. https://doi.org/10.3390/molecules28083343