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Article

High-Throughput Quantitation of Cannabinoids by Liquid Chromatography Triple-Quadrupole Mass Spectrometry

1
Agriculture Victoria Research, AgriBio Centre, AgriBio, Bundoora, VIC 3083, Australia
2
School of Applied Systems Biology, La Trobe University, Bundoora, VIC 3083, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Pierangela Ciuffreda
Molecules 2022, 27(3), 742; https://doi.org/10.3390/molecules27030742
Received: 22 December 2021 / Revised: 17 January 2022 / Accepted: 21 January 2022 / Published: 24 January 2022
(This article belongs to the Section Analytical Chemistry)
The high-throughput quantitation of cannabinoids is important for the cannabis industry. As medicinal products increase, and research into compounds that have pharmacological benefits increase, and the need to quantitate more than just the main cannabinoids becomes more important. This study aims to provide a rapid, high-throughput method for cannabinoid quantitation using a liquid chromatography triple-quadrupole mass spectrometer (LC-QQQ-MS) with an ultraviolet diode array detector (UV-DAD) for 16 cannabinoids: CBDVA, CBDV, CBDA, CBGA, CBG, CBD, THCV, THCVA, CBN, CBNA, THC, Δ8-THC, CBL, CBC, THCA-A and CBCA. Linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, precision, recovery and matrix effect were all evaluated. The validated method was used to determine the cannabinoid concentration of four different Cannabis sativa strains and a low THC strain, all of which have different cannabinoid profiles. All cannabinoids eluted within five minutes with a total analysis time of eight minutes, including column re-equilibration. This was twice as fast as published LC-QQQ-MS methods mentioned in the literature, whilst also covering a wide range of cannabinoid compounds. View Full-Text
Keywords: cannabis; cannabinoids; LC-QQQ-MS; quantitation; high-throughput quantitation; analysis cannabis; cannabinoids; LC-QQQ-MS; quantitation; high-throughput quantitation; analysis
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MDPI and ACS Style

Tran, J.; Elkins, A.C.; Spangenberg, G.C.; Rochfort, S.J. High-Throughput Quantitation of Cannabinoids by Liquid Chromatography Triple-Quadrupole Mass Spectrometry. Molecules 2022, 27, 742. https://doi.org/10.3390/molecules27030742

AMA Style

Tran J, Elkins AC, Spangenberg GC, Rochfort SJ. High-Throughput Quantitation of Cannabinoids by Liquid Chromatography Triple-Quadrupole Mass Spectrometry. Molecules. 2022; 27(3):742. https://doi.org/10.3390/molecules27030742

Chicago/Turabian Style

Tran, Jonathan, Aaron C. Elkins, German C. Spangenberg, and Simone J. Rochfort. 2022. "High-Throughput Quantitation of Cannabinoids by Liquid Chromatography Triple-Quadrupole Mass Spectrometry" Molecules 27, no. 3: 742. https://doi.org/10.3390/molecules27030742

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