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Article

Tripeptide Self-Assembly into Bioactive Hydrogels: Effects of Terminus Modification on Biocatalysis

Chemical & Pharmaceutical Sciences Department, University of Trieste, 34127 Trieste, Italy
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Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Molecules 2021, 26(1), 173; https://doi.org/10.3390/molecules26010173
Received: 9 December 2020 / Revised: 25 December 2020 / Accepted: 28 December 2020 / Published: 31 December 2020
(This article belongs to the Special Issue Hydrogels for Tissue Engineering and Regenerative Medicine)
Bioactive hydrogels based on the self-assembly of tripeptides have attracted great interest in recent years. In particular, the search is active for sequences that are able to mimic enzymes when they are self-organized in a nanostructured hydrogel, so as to provide a smart catalytic (bio)material whose activity can be switched on/off with assembly/disassembly. Within the diverse enzymes that have been targeted for mimicry, hydrolases find wide application in biomaterials, ranging from their use to convert prodrugs into active compounds to their ability to work in reverse and catalyze a plethora of reactions. We recently reported the minimalistic l-His–d-Phe–d-Phe for its ability to self-organize into thermoreversible and biocatalytic hydrogels for esterase mimicry. In this work, we analyze the effects of terminus modifications that mimic the inclusion of the tripeptide in a longer sequence. Therefore, three analogues, i.e., N-acetylated, C-amidated, or both, were synthesized, purified, characterized by several techniques, and probed for self-assembly, hydrogelation, and esterase-like biocatalysis. This work provides useful insights into how chemical modifications at the termini affect self-assembly into biocatalytic hydrogels, and these data may become useful for the future design of supramolecular catalysts for enhanced performance. View Full-Text
Keywords: peptides; hydrogels; biomaterials; self-assembly; chirality; d-amino acids; hydrolase; biocatalysis peptides; hydrogels; biomaterials; self-assembly; chirality; d-amino acids; hydrolase; biocatalysis
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MDPI and ACS Style

Kurbasic, M.; Garcia, A.M.; Viada, S.; Marchesan, S. Tripeptide Self-Assembly into Bioactive Hydrogels: Effects of Terminus Modification on Biocatalysis. Molecules 2021, 26, 173. https://doi.org/10.3390/molecules26010173

AMA Style

Kurbasic M, Garcia AM, Viada S, Marchesan S. Tripeptide Self-Assembly into Bioactive Hydrogels: Effects of Terminus Modification on Biocatalysis. Molecules. 2021; 26(1):173. https://doi.org/10.3390/molecules26010173

Chicago/Turabian Style

Kurbasic, Marina, Ana M. Garcia, Simone Viada, and Silvia Marchesan. 2021. "Tripeptide Self-Assembly into Bioactive Hydrogels: Effects of Terminus Modification on Biocatalysis" Molecules 26, no. 1: 173. https://doi.org/10.3390/molecules26010173

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