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Article

Vitamin C Enhances the Antibacterial Activity of Honey against Planktonic and Biofilm-Embedded Bacteria

1
Laboratory of Apidology and Apitherapy, Department of Microbial Genetics, Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 845 51 Bratislava, Slovakia
2
Department of Microbiology, Faculty of Medicine, Slovak Medical University, Limbova 12, 833 03 Bratislava, Slovakia
3
Regional Health Public Authority, Medercska 39, 945 75 Komarno, Slovakia
4
Protein Science Lab, Department of Biological Sciences, University of Singapore, Singapore 119077, Singapore
*
Author to whom correspondence should be addressed.
Academic Editor: Anwar Rayan
Molecules 2020, 25(4), 992; https://doi.org/10.3390/molecules25040992
Received: 3 February 2020 / Revised: 18 February 2020 / Accepted: 22 February 2020 / Published: 23 February 2020
(This article belongs to the Special Issue Antibiofilm Efficacy of Natural and Natural-Based Products)
Multifactorial antibacterial action is an important feature of honey; however, its bactericidal efficacy against biofilm-embedded bacteria is limited. The aim of this study was to investigate the impact of vitamin C (Vit C) on the antibacterial activity of natural honeys against planktonic as well as biofilm-embedded bacterial pathogens. The antibacterial activity of four honey samples supplemented with Vit C was expressed as the minimum inhibitory concentration (MIC). At sub-MICs, Vit C significantly increased the antibacterial activity of the tested honeys against Pseudomonas aeruginosa in planktonic cultures. However, after supplementation, honeydew honey, the most active honey, was ineffective against Staphylococcus aureus. On the other hand, when 100% honeydew honey was supplemented with Vit C (100 mg/g of honey) in a multispecies wound biofilm model, complete eradication of almost all bacterial isolates, including S. aureus, was observed. Furthermore, a mixture of honey and Vit C was partially effective against Enterococcus faecalis, whereas honey alone exhibited no antibacterial activity against this bacterium. Vit C counteracted hydrogen peroxide in honey solution and, thus, eliminated the major antibacterial compound present in honey. It is likely that a combination of honey with Vit C may trigger the intracellular production of reactive oxygen species in bacterial cells, but the exact cellular mechanisms warrant further investigations. View Full-Text
Keywords: antibacterial activity; hydrogen peroxide; biofilm; wound antibacterial activity; hydrogen peroxide; biofilm; wound
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MDPI and ACS Style

Majtan, J.; Sojka, M.; Palenikova, H.; Bucekova, M.; Majtan, V. Vitamin C Enhances the Antibacterial Activity of Honey against Planktonic and Biofilm-Embedded Bacteria. Molecules 2020, 25, 992. https://doi.org/10.3390/molecules25040992

AMA Style

Majtan J, Sojka M, Palenikova H, Bucekova M, Majtan V. Vitamin C Enhances the Antibacterial Activity of Honey against Planktonic and Biofilm-Embedded Bacteria. Molecules. 2020; 25(4):992. https://doi.org/10.3390/molecules25040992

Chicago/Turabian Style

Majtan, Juraj, Martin Sojka, Helena Palenikova, Marcela Bucekova, and Viktor Majtan. 2020. "Vitamin C Enhances the Antibacterial Activity of Honey against Planktonic and Biofilm-Embedded Bacteria" Molecules 25, no. 4: 992. https://doi.org/10.3390/molecules25040992

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