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Article

The In Vitro Activity of Essential Oils against Helicobacter Pylori Growth and Urease Activity

1
Department of Pharmaceutical Microbiology, Medical University in Lublin, 1 Chodzki Str., 20-093 Lublin, Poland
2
Independent Laboratory of Natural Products Chemistry, Department of Pharmacognosy, Medical University in Lublin, 1 Chodzki Str., 20-093 Lublin, Poland
3
Department of Pharmacognosy, Medical University in Lublin, 1 Chodzki Str., 20-093 Lublin, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Luca Forti
Molecules 2020, 25(3), 586; https://doi.org/10.3390/molecules25030586
Received: 24 December 2019 / Revised: 21 January 2020 / Accepted: 28 January 2020 / Published: 29 January 2020
(This article belongs to the Collection Recent Advances in Flavors and Fragrances)
The anti-H. pylori properties of 26 different commercial essential oils were examined in vitro by MIC (minimal inhibitory concentration) determination for the reference strain H. pylori ATCC 43504. We selected 9 essential oils with different anti-Helicobacter activities and established their phytochemical composition and urease inhibition activities. Phytochemical analysis of the selected essential oils by GC-MS method and antioxidant activity were performed. The phenol red method was used to screen the effect of essential oils on urease activity expressed as IC50 (the half of maximal inhibitory concentration). The most active essential oils, with MIC = 15.6 mg/L, were thyme, lemongrass, cedarwood and lemon balm oils; MIC = 31.3 mg/L—oregano oil; MIC = 62.5 mg/L—tea tree oil; MIC = 125 mg/L—pine needle, lemon and silver fir oils with bactericidal effect. Urease activity was inhibited by these oils with IC50 ranged from 5.3 to > 1049.9 mg/L. The most active was cedarwood oil (IC50 = 5.3 mg/L), inhibiting urease at sub-MIC concentrations (MIC = 15.6 mg/L). The statistical principal component analysis allowed for the division of the oils into three phytochemical groups differing in their anti-H. pylori activity. To summarize, the activity in vitro of the five essential oils silver fir, pine needle, tea tree, lemongrass, and cedarwood oils against H. pylori was found in this paper for the first time. The most active against clinical strains of H. pylori were cedar wood and oregano oils. Moreover, cedarwood oil inhibited the urease activity at subinhibitory concentrations. This essential oil can be regarded as a useful component of the plant preparations supporting the eradication H. pylori therapy. View Full-Text
Keywords: essential oils; chemical analysis; Helicobacter pylori; antibacterial activity essential oils; chemical analysis; Helicobacter pylori; antibacterial activity
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MDPI and ACS Style

Korona-Glowniak, I.; Glowniak-Lipa, A.; Ludwiczuk, A.; Baj, T.; Malm, A. The In Vitro Activity of Essential Oils against Helicobacter Pylori Growth and Urease Activity. Molecules 2020, 25, 586. https://doi.org/10.3390/molecules25030586

AMA Style

Korona-Glowniak I, Glowniak-Lipa A, Ludwiczuk A, Baj T, Malm A. The In Vitro Activity of Essential Oils against Helicobacter Pylori Growth and Urease Activity. Molecules. 2020; 25(3):586. https://doi.org/10.3390/molecules25030586

Chicago/Turabian Style

Korona-Glowniak, Izabela, Anna Glowniak-Lipa, Agnieszka Ludwiczuk, Tomasz Baj, and Anna Malm. 2020. "The In Vitro Activity of Essential Oils against Helicobacter Pylori Growth and Urease Activity" Molecules 25, no. 3: 586. https://doi.org/10.3390/molecules25030586

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