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Article

Covalently Immobilized Battacin Lipopeptide Gels with Activity against Bacterial Biofilms

1
School of Chemical Sciences and the Centre for Green Chemical Science, The University of Auckland, Auckland 1142, New Zealand
2
School of Science, Auckland University of Technology, 34 St. Paul Street, Auckland 1142, New Zealand
3
Department of Biotechnology and Food Engineering, Guangdong Technion Israel Institute of Technology, Shantou 515063, China
4
The MacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New Zealand
*
Author to whom correspondence should be addressed.
Academic Editor: Paul Robert Hansen
Molecules 2020, 25(24), 5945; https://doi.org/10.3390/molecules25245945
Received: 1 November 2020 / Revised: 4 December 2020 / Accepted: 9 December 2020 / Published: 15 December 2020
Novel antibiotic treatments are in increasing demand to tackle life-threatening infections from bacterial pathogens. In this study, we report the use of a potent battacin lipopeptide as an antimicrobial gel to inhibit planktonic and mature biofilms of S. aureus and P. aeruginosa. The antimicrobial gels were made by covalently linking the N-terminal cysteine containing lipopeptide (GZ3.163) onto the polyethylene glycol polymer matrix and initiating gelation using thiol-ene click chemistry. The gels were prepared both in methanol and in water and were characterised using rheology, Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). Antibacterial and antibiofilm analyses revealed that the gels prepared in methanol have better antibacterial and antibiofilm activity. Additionally, a minimum peptide content of 0.5 wt% (relative to polymer content) is required to successfully inhibit the planktonic bacterial growth and disperse mature biofilms of P. aeruginosa and S. aureus. The antibacterial activity of these lipopeptide gels is mediated by a contact kill mechanism of action. The gels are non-haemolytic against mouse red blood cells and are non-cytotoxic against human dermal fibroblasts. Findings from this study show that battacin lipopeptide gels have the potential to be developed as novel topical antibacterial agents to combat skin infections, particularly caused by S. aureus. View Full-Text
Keywords: antimicrobial lipopeptides; antimicrobial hydrogels; bacterial biofilm inhibition; non haemolytic; non-cytotoxic antimicrobial lipopeptides; antimicrobial hydrogels; bacterial biofilm inhibition; non haemolytic; non-cytotoxic
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MDPI and ACS Style

De Zoysa, G.H.; Wang, K.; Lu, J.; Hemar, Y.; Sarojini, V. Covalently Immobilized Battacin Lipopeptide Gels with Activity against Bacterial Biofilms. Molecules 2020, 25, 5945. https://doi.org/10.3390/molecules25245945

AMA Style

De Zoysa GH, Wang K, Lu J, Hemar Y, Sarojini V. Covalently Immobilized Battacin Lipopeptide Gels with Activity against Bacterial Biofilms. Molecules. 2020; 25(24):5945. https://doi.org/10.3390/molecules25245945

Chicago/Turabian Style

De Zoysa, Gayan H., Kelvin Wang, Jun Lu, Yacine Hemar, and Vijayalekshmi Sarojini. 2020. "Covalently Immobilized Battacin Lipopeptide Gels with Activity against Bacterial Biofilms" Molecules 25, no. 24: 5945. https://doi.org/10.3390/molecules25245945

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