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Qualitative and Quantitative Analysis of Regional Cerebral Free Fatty Acids in Rats Using the Stable Isotope Labeling Liquid Chromatography–Mass Spectrometry Method

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State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China
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University of Chinese Academy of Sciences, Beijing 100049, China
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Department of Chemistry, Wuhan University, Wuhan 430072, China
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Department of Chemistry, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan 64200, Pakistan
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Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan 430072, China
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School of Human and Social Sciences, University of West London, Middlesex TW89GA, UK
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Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China
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Authors to whom correspondence should be addressed.
Molecules 2020, 25(21), 5163; https://doi.org/10.3390/molecules25215163
Received: 6 September 2020 / Revised: 24 October 2020 / Accepted: 28 October 2020 / Published: 6 November 2020
(This article belongs to the Section Analytical Chemistry)
Free fatty acids serve as important bioactive molecules in the brain. They are involved in message transfer in the brain. There are many reports available in the literature regarding the role of cerebral fatty acids in message transfer; however, most of the studies are mainly focused on limited fatty acid species or only a few specific brain regions. To understand the relationship between cerebral functions and free fatty acids, it is necessary to investigate the distribution of the free fatty acids among different regions in the whole brain. In this study, free fatty acids were extracted from different brain regions and analyzed qualitatively and quantitatively using the stable isotopic labeling liquid chromatography–mass spectrometry approach. In total, 1008 potential free fatty acids were detected in the whole brain out of which 38 were found to be commonly present in all brain regions. Among different brain regions, the highest and the smallest amounts of potential free fatty acids were detected in the olfactory bulb and cerebellum, respectively. From a statistical point of view, 4-methyl-2-oxovaleric acid, cis-11, 14-eicosadienoic acid, tridecanoic acid, myristic acid, nonadecanoic acid, and arachidic acid were found to significantly vary among the four different brain regions (olfactory bulb, occipital lobe, hippocampus, and cerebellum). The variation in the composition of free fatty acids among different brain regions may be very important for investigating the relationship between free fatty acids and functions of cerebral regions. View Full-Text
Keywords: fatty acids; brain; liquid chromatography–mass spectrometry method; stable isotope labeling; regions fatty acids; brain; liquid chromatography–mass spectrometry method; stable isotope labeling; regions
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MDPI and ACS Style

Hu, T.; Zhu, Q.; Hu, Y.; Kamal, G.M.; Feng, Y.; Manyande, A.; Wang, J.; Xu, F. Qualitative and Quantitative Analysis of Regional Cerebral Free Fatty Acids in Rats Using the Stable Isotope Labeling Liquid Chromatography–Mass Spectrometry Method. Molecules 2020, 25, 5163. https://doi.org/10.3390/molecules25215163

AMA Style

Hu T, Zhu Q, Hu Y, Kamal GM, Feng Y, Manyande A, Wang J, Xu F. Qualitative and Quantitative Analysis of Regional Cerebral Free Fatty Acids in Rats Using the Stable Isotope Labeling Liquid Chromatography–Mass Spectrometry Method. Molecules. 2020; 25(21):5163. https://doi.org/10.3390/molecules25215163

Chicago/Turabian Style

Hu, Ting, Quanfei Zhu, Yuning Hu, Ghulam M. Kamal, Yuqi Feng, Anne Manyande, Jie Wang, and Fuqiang Xu. 2020. "Qualitative and Quantitative Analysis of Regional Cerebral Free Fatty Acids in Rats Using the Stable Isotope Labeling Liquid Chromatography–Mass Spectrometry Method" Molecules 25, no. 21: 5163. https://doi.org/10.3390/molecules25215163

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