Next Article in Journal
New Zileuton-Hydroxycinnamic Acid Hybrids: Synthesis and Structure-Activity Relationship towards 5-Lipoxygenase Inhibition
Next Article in Special Issue
Quantification of Cooking Method Effect on COP Content in Meat Types Using Triple Quadrupole GC-MS/MS
Previous Article in Journal
The Effects of Tannins in Monogastric Animals with Special Reference to Alternative Feed Ingredients
Previous Article in Special Issue
In Silico Molecular Study of Tryptophan Bitterness
Open AccessFeature PaperArticle

In Vitro Antimicrobial and Antioxidant Activities of Lactobacillus coryniformis BCH-4 Bioactive Compounds and Determination of their Bioprotective Effects on Nutritional Components of Maize (Zea mays L.)

1
Department of Biochemistry, Government College University Faisalabad (GCUF), Allama Iqbal Road, Faisalabad 38000, Pakistan
2
Department of Bioinformatics and Biotechnology, Government College University Faisalabad (GCUF), Allama Iqbal Road, Faisalabad 38000, Pakistan
3
Department of Chemistry, Government College University Faisalabad (GCUF), Allama Iqbal Road, Faisalabad 38000, Pakistan
4
Botany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia
5
Mycology and Plant Disease Survey Department, Plant Pathology Research Institute, ARC, Giza 12511, Egypt
6
Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia
7
Botany and Microbiology Department, Faculty of Science, Cairo University, Giza 12613, Egypt
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Panagiotis Zoumpoulakis
Molecules 2020, 25(20), 4685; https://doi.org/10.3390/molecules25204685
Received: 10 September 2020 / Revised: 6 October 2020 / Accepted: 8 October 2020 / Published: 14 October 2020
(This article belongs to the Special Issue Bioactive Compounds with Applications in Health and Food)
Lactic acid bacteria (LAB) can synthesize antimicrobial compounds (AMCs) with nutritional and bioprotective properties in crops and food products. In the current study, AMCs of Lactobacillus coryniformis BCH-4 were evaluated to control fungal spoilage in maize grains. On maize grains treated with 75%–100% (v/v) concentrated AMCs, no fungal growth was observed even after 72 h of Aspergillus flavus inoculation. Proximate analysis of treatments A1 (raw grains), A2 (A. flavus inoculated grains) and A3 (A. flavus + AMCs inoculated grains) revealed that moisture was significantly (p ≤ 0.05) high in A2 than A3 and A1. Meanwhile, protein, fat, fiber and ash contents were significantly decreased in A2 compared to A1 and A3. Moreover, β-carotene contents were not statistically different between A1 and A3, while in A2 it was significantly decreased. HPLC analysis revealed the presence of 2-oxopropanoic acid, 2-hydroxypropane-1,2,3-tricarboxylic acid, 2-hydroxybutanedioic acid, 2-hydroxypropanoic acid, propanedioic acid and butanedioic acid, which also showed antifungal activity against Aspergillus flavus. FTIR spectroscopy revealed the presence of hydroxyl, carbonyl and ester-groups along with organic and fatty acids, thereby indicating their participation in inhibitory action. Furthermore, the AMCs were found to be a good alternative to chemical preservatives, thereby not only preserving the nutritive qualities but increasing the shelf life as well. View Full-Text
Keywords: Lactobacillus coryniformis; antimicrobial compounds; maize (Zea mays L.); HPLC; FTIR Lactobacillus coryniformis; antimicrobial compounds; maize (Zea mays L.); HPLC; FTIR
Show Figures

Graphical abstract

MDPI and ACS Style

Salman, M.; Tariq, A.; Ijaz, A.; Naheed, S.; Hashem, A.; Abd_Allah, E.F.; Soliman, M.H.; Javed, M.R. In Vitro Antimicrobial and Antioxidant Activities of Lactobacillus coryniformis BCH-4 Bioactive Compounds and Determination of their Bioprotective Effects on Nutritional Components of Maize (Zea mays L.). Molecules 2020, 25, 4685.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop