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Article

Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell Injury

1
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung City 807, Taiwan
2
Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan
3
Department of Sport Performance, National Taiwan University of Sport, Taichung 404, Taiwan
4
Division of Plastic and Reconstructive Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei 112, Taiwan
5
Department of Pharmacy and Master Program, Tajen University, Pingtung 907, Taiwan
6
Drug Development and Value Creation Research Center, Kaohsiung Medical University, Kaohsiung 807, Taiwan
*
Authors to whom correspondence should be addressed.
Molecules 2020, 25(14), 3267; https://doi.org/10.3390/molecules25143267
Received: 3 June 2020 / Revised: 9 July 2020 / Accepted: 15 July 2020 / Published: 17 July 2020
Glucocorticoids are widely used anti-inflammatory drugs in clinical settings. However, they can induce skeletal muscle atrophy by reducing fiber cross-sectional area and myofibrillar protein content. Studies have proven that antioxidants can improve glucocorticoid-induced skeletal muscle atrophy. Quercetin is a potent antioxidant flavonoid widely distributed in fruits and vegetables and has shown protective effects against dexamethasone-induced skeletal muscle atrophy. In this study, we demonstrated that dexamethasone significantly inhibited cell growth and induced cell apoptosis by stimulating hydroxyl free radical production in C2C12 skeletal muscle cells. Our results evidenced that quercetin increased C2C12 skeletal cell viability and exerted antiapoptotic effects on dexamethasone-treated C2C12 cells by regulating mitochondrial membrane potential (ΔΨm) and reducing oxidative species. Quercetin can protect against dexamethasone-induced muscle atrophy by regulating the Bax/Bcl-2 ratio at the protein level and abnormal ΔΨm, which leads to the suppression of apoptosis. View Full-Text
Keywords: apoptosis; antioxidant; C2C12 skeletal muscle cells; dexamethasone; mitochondrial membrane potential (ΔΨm); quercetin apoptosis; antioxidant; C2C12 skeletal muscle cells; dexamethasone; mitochondrial membrane potential (ΔΨm); quercetin
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MDPI and ACS Style

Chen, C.; Yang, J.-S.; Lu, C.-C.; Chiu, Y.-J.; Chen, H.-C.; Chung, M.-I.; Wu, Y.-T.; Chen, F.-A. Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell Injury. Molecules 2020, 25, 3267. https://doi.org/10.3390/molecules25143267

AMA Style

Chen C, Yang J-S, Lu C-C, Chiu Y-J, Chen H-C, Chung M-I, Wu Y-T, Chen F-A. Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell Injury. Molecules. 2020; 25(14):3267. https://doi.org/10.3390/molecules25143267

Chicago/Turabian Style

Chen, Chun, Jai-Sing Yang, Chi-Cheng Lu, Yu-Jen Chiu, Hung-Che Chen, Mei-Ing Chung, Yu-Tse Wu, and Fu-An Chen. 2020. "Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell Injury" Molecules 25, no. 14: 3267. https://doi.org/10.3390/molecules25143267

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