Next Article in Journal
Alkyl and Aryl Derivatives Based on p-Coumaric Acid Modification and Inhibitory Action against Leishmania braziliensis and Plasmodium falciparum
Next Article in Special Issue
Influence of PVA Molecular Weight and Concentration on Electrospinnability of Birch Bark Extract-Loaded Nanofibrous Scaffolds Intended for Enhanced Wound Healing
Previous Article in Journal
The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark
Open AccessArticle

Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential

1
Unit of Basic and Applied Biosciences, Faculty of Bioscience and Agro-Food and Environmental Technology, University of Teramo, 64100 Teramo, Italy
2
Department of Biomaterials, INNOVENT e. V., 07745 Jena, Germany
3
Department of Surface Engineering, INNOVENT e. V., 07745 Jena, Germany
*
Author to whom correspondence should be addressed.
Equal senior authors.
Academic Editor: Suman Sinha Ray
Molecules 2020, 25(14), 3176; https://doi.org/10.3390/molecules25143176
Received: 8 June 2020 / Revised: 6 July 2020 / Accepted: 9 July 2020 / Published: 11 July 2020
(This article belongs to the Special Issue Polymer Scaffolds for Biomedical Applications 2020)
Electrospun PLGA microfibers with adequate intrinsic physical features (fiber alignment and diameter) have been shown to boost teno-differentiation and may represent a promising solution for tendon tissue engineering. However, the hydrophobic properties of PLGA may be adjusted through specific treatments to improve cell biodisponibility. In this study, electrospun PLGA with highly aligned microfibers were cold atmospheric plasma (CAP)-treated by varying the treatment exposure time (30, 60, and 90 s) and the working distance (1.3 and 1.7 cm) and characterized by their physicochemical, mechanical and bioactive properties on ovine amniotic epithelial cells (oAECs). CAP improved the hydrophilic properties of the treated materials due to the incorporation of new oxygen polar functionalities on the microfibers’ surface especially when increasing treatment exposure time and lowering working distance. The mechanical properties, though, were affected by the treatment exposure time where the optimum performance was obtained after 60 s. Furthermore, CAP treatment did not alter oAECs’ biocompatibility and improved cell adhesion and infiltration onto the microfibers especially those treated from a distance of 1.3 cm. Moreover, teno-inductive potential of highly aligned PLGA electrospun microfibers was maintained. Indeed, cells cultured onto the untreated and CAP treated microfibers differentiated towards the tenogenic lineage expressing tenomodulin, a mature tendon marker, in their cytoplasm. In conclusion, CAP treatment on PLGA microfibers conducted at 1.3 cm working distance represent the optimum conditions to activate PLGA surface by improving their hydrophilicity and cell bio-responsiveness. Since for tendon tissue engineering purposes, both high cell adhesion and mechanical parameters are crucial, PLGA treated for 60 s at 1.3 cm was identified as the optimal construct. View Full-Text
Keywords: aligned microfibers; amniotic epithelial stem cells; cold atmospheric plasma; electrospinning; exposure time; PLGA; tendon tissue engineering; working distance aligned microfibers; amniotic epithelial stem cells; cold atmospheric plasma; electrospinning; exposure time; PLGA; tendon tissue engineering; working distance
Show Figures

Figure 1

MDPI and ACS Style

El Khatib, M.; Mauro, A.; Wyrwa, R.; Di Mattia, M.; Turriani, M.; Di Giacinto, O.; Kretzschmar, B.; Seemann, T.; Valbonetti, L.; Berardinelli, P.; Schnabelrauch, M.; Barboni, B.; Russo, V. Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential. Molecules 2020, 25, 3176.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop