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On the Reversible and Irreversible Inhibition of Rhodesain by Curcumin
Article

Neuroprotective Effects of Tetrahydrocurcumin against Glutamate-Induced Oxidative Stress in Hippocampal HT22 Cells

1
College of Korean Medicine, Gachon University, Seongnam 13120, Korea
2
Natural Products Research Institute, Korea Institute of Science and Technology Gangneung 25451, Korea
3
Department of Food and Nutrition, Gachon University, Seongnam 13120, Korea
*
Authors to whom correspondence should be addressed.
Academic Editors: Derek J. McPhee and Cristobal De Los Rios
Molecules 2020, 25(1), 144; https://doi.org/10.3390/molecules25010144
Received: 27 November 2019 / Revised: 23 December 2019 / Accepted: 28 December 2019 / Published: 30 December 2019
(This article belongs to the Special Issue Drugs Modulating Calcium Signaling)
In the central nervous system, glutamate is a major excitable neurotransmitter responsible for many cellular functions. However, excessive levels of glutamate induce neuronal cell death via oxidative stress during acute brain injuries as well as chronic neurodegenerative diseases. The present study was conducted to examine the effect of tetrahydrocurcumin (THC), a major secondary metabolite of curcumin, and its possible mechanism against glutamate-induced cell death. We prepared THC using curcumin isolated from Curcuma longa (turmeric) and demonstrated the protective effect of THC against glutamate-induced oxidative stress in HT22 cells. THC abrogated glutamate-induced HT22 cell death and showed a strong antioxidant effect. THC also significantly reduced intracellular calcium ion increased by glutamate. Additionally, THC significantly reduced the accumulation of intracellular oxidative stress induced by glutamate. Furthermore, THC significantly diminished apoptotic cell death indicated by annexin V-positive in HT22 cells. Western blot analysis indicated that the phosphorylation of mitogen-activated protein kinases including c-Jun N-terminal kinase, extracellular signal-related kinases 1/2, and p38 by glutamate was significantly diminished by treatment with THC. In conclusion, THC is a potent neuroprotectant against glutamate-induced neuronal cell death by inhibiting the accumulation of oxidative stress and phosphorylation of mitogen-activated protein kinases. View Full-Text
Keywords: glutamate; oxidative stress; mitogen-activated protein kinase; Ca2+; tetrahydrocurcumin; HT22 cells glutamate; oxidative stress; mitogen-activated protein kinase; Ca2+; tetrahydrocurcumin; HT22 cells
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MDPI and ACS Style

Park, C.-H.; Song, J.H.; Kim, S.-N.; Lee, J.H.; Lee, H.-J.; Kang, K.S.; Lim, H.-H. Neuroprotective Effects of Tetrahydrocurcumin against Glutamate-Induced Oxidative Stress in Hippocampal HT22 Cells. Molecules 2020, 25, 144. https://doi.org/10.3390/molecules25010144

AMA Style

Park C-H, Song JH, Kim S-N, Lee JH, Lee H-J, Kang KS, Lim H-H. Neuroprotective Effects of Tetrahydrocurcumin against Glutamate-Induced Oxidative Stress in Hippocampal HT22 Cells. Molecules. 2020; 25(1):144. https://doi.org/10.3390/molecules25010144

Chicago/Turabian Style

Park, Chang-Hyun, Ji H. Song, Su-Nam Kim, Ji H. Lee, Hae-Jeung Lee, Ki S. Kang, and Hyung-Ho Lim. 2020. "Neuroprotective Effects of Tetrahydrocurcumin against Glutamate-Induced Oxidative Stress in Hippocampal HT22 Cells" Molecules 25, no. 1: 144. https://doi.org/10.3390/molecules25010144

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