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Open AccessArticle

Bunias erucago L.: Glucosinolate Profile and In Vitro Biological Potential

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Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia
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School of Medicine, University of Split, Šoltanska 2, 21000 Split, Croatia
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Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia
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Department of Chemistry, University of Split, Faculty of Science, Ruđera Boškovića 33, 21000 Split, Croatia
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Department of Biology, University of Split, Faculty of Science, Ruđera Boškovića 33, 21000 Split, Croatia
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Department of Food Technology and Biotechnology, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia
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Author to whom correspondence should be addressed.
Molecules 2019, 24(4), 741; https://doi.org/10.3390/molecules24040741
Received: 9 January 2019 / Revised: 13 February 2019 / Accepted: 14 February 2019 / Published: 19 February 2019
(This article belongs to the Section Natural Products Chemistry)
Bunias erucago belongs to the Brassicaceae family, which represents a forgotten crop of the Euro-Mediterranean area. The aim of the present study was to determine the glucosinolate profile in different plant parts and biological properties (antioxidant, anticholinesterase, and cytotoxic activities) of the isolates containing glucosinolate breakdown products. The chemical profiles were determined by using HPLC-PDA-MS/MS of desulfoglucosinolates and GC-MS of glucosinolate degradation products. The analysis of B. erucago showed the presence of seven glucosinolates: gluconapin (1), glucoraphasatin (2), glucoraphenin (3), glucoerucin (4), glucoraphanin (5), glucotropaeolin (6), and glucosinalbin (7). The total glucosinolate content ranged from 7.0 to 14.6 µmol/g of dry weight, with the major glucosinolate glucosinalbin in all parts. The antioxidant activity of all volatile isolates was not notable. At a tested concentration of 227 μg/mL, flower hydro-distillate (FH) showed good AChE inhibition, i.e., 40.9%, while root hydro-distillate (RH) had good activity against BChE, i.e., 54.3%. FH showed the best activity against both tested human bladder cancer cell lines, i.e., against T24 after 72 h, which have IC50 of 16.0 μg/mL, and against TCCSUP after 48 h with IC50 of 7.8 μg/mL, and can be considered as highly active. On the other hand, RH showed weak activity against tested cancer cells. View Full-Text
Keywords: Bunias erucago L.; glucosinolates; desulfoglucosinolates; isothiocyanates; nitriles; anti-oxidation; cholinesterase inhibition; cytotoxic activity Bunias erucago L.; glucosinolates; desulfoglucosinolates; isothiocyanates; nitriles; anti-oxidation; cholinesterase inhibition; cytotoxic activity
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Blažević, I.; Đulović, A.; Čikeš Čulić, V.; Burčul, F.; Ljubenkov, I.; Ruščić, M.; Generalić Mekinić, I. Bunias erucago L.: Glucosinolate Profile and In Vitro Biological Potential. Molecules 2019, 24, 741.

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