Next Article in Journal
Evaluating the Anti-cancer Efficacy of a Synthetic Curcumin Analog on Human Melanoma Cells and Its Interaction with Standard Chemotherapeutics
Previous Article in Journal
Stable Deuterium Labeling of Histidine-Rich Lysine-Based Dendrimers
Previous Article in Special Issue
Long-Lasting Anti-Inflammatory and Antinociceptive Effects of Acute Ammonium Glycyrrhizinate Administration: Pharmacological, Biochemical, and Docking Studies
Article Menu
Issue 13 (July-1) cover image

Export Article

Open AccessArticle

Nardochinoid B Inhibited the Activation of RAW264.7 Macrophages Stimulated by Lipopolysaccharide through Activating the Nrf2/HO-1 Pathway

1
Faculty of Chinese Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China
2
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China
3
College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
4
ICBAS-Laboratory of Applied Physiology, Abel Salazar Institute of Biomedical Sciences, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
5
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China
6
College of Pharmacy, Hunan University of Chinese Medicine, Changsha 418000, China
7
Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People’s Republic of China, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Francesco Maione
Molecules 2019, 24(13), 2482; https://doi.org/10.3390/molecules24132482
Received: 15 May 2019 / Revised: 15 June 2019 / Accepted: 5 July 2019 / Published: 6 July 2019
  |  
PDF [2130 KB, uploaded 6 July 2019]
  |     |  

Abstract

Nardochinoid B (NAB) is a new compound isolated from Nardostachys chinensis. Although our previous study reported that the NAB suppressed the production of nitric oxide (NO) in lipopolysaccharide (LPS)-activated RAW264.7 cells, the specific mechanisms of anti-inflammatory action of NAB remains unknown. Thus, we examined the effects of NAB against LPS-induced inflammation. In this study, we found that NAB suppressed the LPS-induced inflammatory responses by restraining the expression of inducible nitric oxide synthase (iNOS) proteins and mRNA instead of cyclooxygenase-2 (COX-2) protein and mRNA in RAW264.7 cells, implying that NAB may have lower side effects compared with nonsteroidal anti-inflammatory drugs (NSAIDs). Besides, NAB upregulated the protein and mRNA expressions of heme oxygenase (HO)-1 when it exerted its anti-inflammatory effects. Also, NAB restrained the production of NO by increasing HO-1 expression in LPS-stimulated RAW264.7 cells. Thus, it is considered that the anti-inflammatory effect of NAB is associated with an induction of antioxidant protein HO-1, and thus NAB may be a potential HO-1 inducer for treating inflammatory diseases. Moreover, our study found that the inhibitory effect of NAB on NO is similar to that of the positive drug dexamethasone, suggesting that NAB has great potential for developing new drugs in treating inflammatory diseases. View Full-Text
Keywords: Nardostachys chinensis; nardochinoid B; nitric oxide; inducible nitric oxide synthase; heme oxygenase-1 Nardostachys chinensis; nardochinoid B; nitric oxide; inducible nitric oxide synthase; heme oxygenase-1
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Yao, Y.-D.; Shen, X.-Y.; Machado, J.; Luo, J.-F.; Dai, Y.; Lio, C.-K.; Yu, Y.; Xie, Y.; Luo, P.; Liu, J.-X.; Yao, X.-S.; Liu, Z.-Q.; Zhou, H. Nardochinoid B Inhibited the Activation of RAW264.7 Macrophages Stimulated by Lipopolysaccharide through Activating the Nrf2/HO-1 Pathway. Molecules 2019, 24, 2482.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top