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Open AccessArticle

Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein

by 1,2, 1,2, 1,2, 1,2, 1,2 and 1,2,*
1
Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, No 7, Nanhai Road, Qingdao 266071, China
2
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, No 1, Wenhai Road, Qingdao 266237, China
*
Author to whom correspondence should be addressed.
Academic Editors: María Pilar Almajano and Michael H. Gordon
Molecules 2019, 24(10), 1946; https://doi.org/10.3390/molecules24101946
Received: 12 April 2019 / Revised: 14 May 2019 / Accepted: 18 May 2019 / Published: 21 May 2019
(This article belongs to the Special Issue Antioxidants Chemistry and Applications)
The aim of this study was to isolate and purify antioxidative peptides from Pacific herring (Clupea pallasii) protein. Five enzymes (pepsin, trypsin, papain, flavourzyme, and neutrase) were used for protein hydrolysis, and Pacific herring protein hydrolysates (PHPH) were separated by ultrafiltration. The fraction with the molecular weight below 3500 Da exhibited the highest in vitro antioxidant activities and cellular antioxidant activity. The PHPH was isolated and purified by consecutive chromatographic methods including gel filtration chromatography and reverse high-performance liquid chromatography (RP-HPLC). The purified antioxidant peptides were identified as Leu-His-Asp-Glu-Leu-Thr (MW = 726.35 Da) and Lys-Glu-Glu-Lys-Phe-Glu (MW = 808.40 Da), and the IC50 values of cellular antioxidant activity were 1.19 ± 0.05 mg/mL and 1.04 ± 0.06 mg/mL. The results demonstrate that is possible to produce natural antioxidative peptides from Pacific herring protein via enzymatic hydrolysis and purification. View Full-Text
Keywords: pacific herring; protein hydrolysate; peptides; antioxidant activity; purification pacific herring; protein hydrolysate; peptides; antioxidant activity; purification
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MDPI and ACS Style

Wang, X.; Yu, H.; Xing, R.; Liu, S.; Chen, X.; Li, P. Preparation and Identification of Antioxidative Peptides from Pacific Herring (Clupea pallasii) Protein. Molecules 2019, 24, 1946.

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