Search Results (4,634)

Fungi contribute to ecosystem function through nutrient cycling and decomposition but may be affected by major disturbances such as fire. Some ecosystems are fire-adapted, such as prairies which require cyclical burning to mitigate woody plant encroachment and reduce litter. While fire suppresses fire-sensitive fungi, pyrophilous fungi may continue providing ecosystem functions. Using litter bags, we measured the litter decomposition at three prairies with unburned and burned sections, and we used Illumina sequencing to examine litter communities. We hypothesized that (H1) decomposition would be higher at unburned sites than burned, (H2) increased decomposition at unburned sites would be correlated with higher overall saprotroph diversity, with a lower diversity in autoclaved samples, and (H3) pyrophilous fungal diversity would be higher at burned sites and overall higher in autoclaved samples. H1 was not supported; decomposition was unaffected by burn treatments. H2 and H3 were somewhat supported; saprotroph diversity was lowest in autoclaved litter at burned sites, but pyrophilous fungal diversity was the highest. Pyrophilous fungal diversity significantly contributed to litter decomposition rates, while saprotroph diversity did not. Our findings indicate that fire-adapted prairies host a suite of pyrophilous saprotrophic fungi, and that these fungi play a primary role in litter decomposition post-fire when other fire-sensitive fungal saprotrophs are less abundant. Full article

In the summer of 2022, central and eastern China experienced prolonged extreme high temperatures and severe drought, leading to significant economic losses. To gain a more profound understanding of this drought event and furnish a reference for forecasting similar events in the future, this study examines the circulation anomalies associated with the drought. Employing a diagnostic method focused on temperature and moisture anomalies, this study introduces a novel approach to quantify and compare the relative significance of moisture transport and warm air dynamics in contributing to the drought. This study examines the atmospheric circulation anomalies linked to the drought event and compares the relative contributions of water vapor transport and warm air activity in causing the drought, using two parameters defined in the paper. The results show the following: (1) The West Pacific Subtropical High (WPSH) was more intense than usual and extended westward, consistently controlling the Yangtze River Basin. Simultaneously, the polar vortex area was smaller and weaker, the South Asian High area was larger and stronger, and it shifted eastward. These factors collectively led to weakened water vapor transport conditions and prevailing subsiding air motions in the Yangtze River Basin, causing frequent high temperatures. (2) By defining I_q and I_t to represent the contributions of moisture and temperature to precipitation, we found that the drought event in the Yangtze River Basin was driven by both reduced moisture supplies in the lower troposphere and higher-than-normal temperatures, with temperature playing a dominant role. Full article

The ecological impact of crown-of-thorns starfish (CoTS; Acanthaster spp.) on coral reefs is intrinsically linked to their feeding behaviour. Management thresholds designed to mitigate coral loss driven by elevated densities of crown-of-thorns starfish rely on accurate estimates of individual feeding rates. In this study, structure-from-motion photogrammetry and intensive tracking of adult Pacific CoTS over an extended survey period were used to generate three-dimensional, high-resolution estimates of daily feeding rates. Our findings revealed substantial variation in the areal extent of coral consumed, both across consecutive days and among individuals. Notably, CoTS did not feed consistently; feeding occurred on 65% of observation days, with 2–3 days periods of inactivity common. Despite this variability, mean daily feeding rates aligned with previous studies (1.35 coral colonies d⁻¹; 198.4 cm² day⁻¹ planar area, and 998.83 cm² day⁻¹ three-dimensional surface area). Across all tracked individuals (n = 8), feeding was recorded on 17 coral genera; however, Acropora alone accounted for 51% of colonies consumed and contributed 82% of the total three-dimensional surface area ingested during the survey period. This highlights the disproportionately large feeding yield derived from Acropora-dominated diets and raises important questions about how future declines in Acropora cover may impact CoTS feeding success and energetic intake. Full article

The use of eco-organic ingredients as a source of protein in aquaculture diets needs important attention due to the growing demand for organic seafood products. The present study evaluated the effects of fish meal substitution by different organic ingredients on the growth, body composition, retention efficiency, enzyme activity, and nutrient digestibility of white shrimp Penaeus vannamei. The four dietary formulations tested were formulated with organic ingredients and the fish meal was replaced by the following organic protein meals: Iberian pig viscera meal (PIG), trout by-product meal (TRO), insect meal (FLY), and organic vegetable meal (WHT), in addition to a control diet (CON) that included 15% fish meal. A growth trial was carried out for 83 days, raising 1 g shrimp to commercial size (20 g). Shrimp were stocked at 167 shrimp/m³ (15 individuals per 90 L tank). The results showed that the growth obtained by shrimp fed with TRO (19.27 g) and PIG (19.35 g) were similar in weight gain to the control diet (20.76 g), while FLY (16.04 g) and WHT (16.73 g) meals resulted in a significant lower final weight. The FLY diet showed significantly lower protein digestibility (68.89%) compared to the CON, PIG, TRO, and WHT diets, and significantly higher trypsin activity (0.17 mU/g) compared to shrimp fed with the PIG, TRO, and WHT diets. Shrimp fed with WHT have a significantly lower body weight percentage of protein (19.69%) than shrimp fed with the WHT and TRO diets, and some significant differences in dietary aminoacidic levels affecting amino acid body composition. These results indicate that Iberian pig viscera and trout by-product meal can successfully replace fish meal in Pacific white shrimp aquaculture. Full article

The spatial variation of forest ecosystem services at regional scales remains poorly understood, and few studies have explicitly analyzed how ecosystem services are distributed across different forest management types. This study assessed the spatial overlap between forest management types and ecosystem service hotspots in the Southeastern United States (SEUS) and the Pacific Northwest (PNW) forests. We used the InVEST suite of tools and GIS to quantify carbon storage and water yield. Carbon storage was estimated, stratified by forest group and age class, and literature-based biomass pool values were applied. Average annual water yield and its temporal changes (2001–2020) were modeled using the annual water yield model, incorporating precipitation, potential evapotranspiration, vegetation type, and soil characteristics. Ecosystem service outputs were classified to identify hotspot zones (top 20%) and to evaluate the synergies and tradeoffs between these services. Hotspots were then overlaid with forest management maps to examine their distribution across management types. We found that only 2% of the SEUS and 11% of the PNW region were simultaneous hotspots for both services. In the SEUS, ecological and preservation forest management types showed higher efficiency in hotspot allocation, while in PNW, production forestry contributed relatively more to hotspot areas. These findings offer valuable insights for decision-makers and forest managers seeking to preserve the multiple benefits that forests provide at regional scales. Full article

The biopharmaceutical industry is undergoing a fundamental transformation from traditional batch manufacturing to continuous manufacturing (CM) for recombinant drugs and biosimilars, driven by regulatory support through the International Council for Harmonization (ICH) Q13 guidance and compelling economic advantages. This comprehensive review examines the technical, economic, and regulatory aspects of implementing continuous manufacturing specifically for recombinant protein production and biosimilar development, synthesizing validated data from peer-reviewed research, regulatory sources, and global implementation case studies. The analysis demonstrates that continuous manufacturing offers substantial benefits, including a reduced equipment footprint of up to 70%, a 3- to 5-fold increase in volumetric productivity, enhanced product quality consistency, and facility cost reductions of 30–50% compared to traditional batch processes. Leading biomanufacturers across North America, Europe, and the Asia–Pacific region are successfully integrating perfusion upstream processes with connected downstream bioprocesses, enabling the fully end-to-end continuous manufacture of biopharmaceuticals with demonstrated commercial viability. The regulatory framework has been comprehensively established through ICH Q13 guidance and region-specific implementations across the FDA, EMA, PMDA, and emerging market authorities. This review provides a critical analysis of advanced technologies, including single-use perfusion bioreactors, continuous chromatography systems, real-time process analytical technology, and Industry 4.0 integration strategies. The economic modeling presents favorable return-on-investment profiles, accompanied by a detailed analysis of global market dynamics, regional implementation patterns, and supply chain integration opportunities. Full article

Motor vehicle crashes are a persistent cause of unintentional deaths in the United States. Scholarship on how manmade interventions and natural phenomena interact to effectuate such calamitous outcomes is longstanding. One manmade intervention of interest in the literature is daylight saving time (DST). Unfortunately, results on how the natural phenomena attributable to DST interact with driver behavior are inconsistent. To advance knowledge on DST-safety interactions, this study adopts a multilevel model approach to fatal motor vehicle crash outcomes in the contiguous United States. Results from a national analysis contextualize results from zonal analyses to unmask within- and between-time zone differences in DST-safety interactions. In the national analysis, motor vehicle crash fatalities decrease somewhat during DST (−0.10%). In the zonal analyses, motor vehicle crash fatalities decrease more so in the Central and Eastern time zones (−2.00% and −2.00%, respectively), but increase somewhat in the Pacific and Mountain time zones (+0.30%) during DST. The spatiotemporal context of the national analysis highlights specific policy implications from the zonal analyses to decrease the lethality of motor vehicle crashes. Specifically, interdictions to target alcohol and/or drug involvement in the northern latitudes of the Pacific and Mountain time zones during DST, the Central time zone at dawn or dusk before or after DST, and the northern latitudes in the Eastern time zone before or after DST are important. Generally, national DST-safety benefits mask zonal DST-safety costs in the Pacific and Mountain time zones. Full article

The geographical response of western North Pacific subtropical high (SH) to environmental conditions such as the El Niño-Southern Oscillation (ENSO) and global warming has been one of the main concerns with respect to extreme events induced by tropical convections. By considering observed outgoing longwave radiation (OLR) as the strength of subtropical high, this study attempts to further understand the geographical response of SH strength to ENSO and global warming. Here, “SH strength” is defined as the inhibition of regional convections under SH environment. A meridional seesaw pattern among SH strength anomalies is found at 130°–175° E. In addition, the La Niña environment with weaker convections at lower latitudes is characterized by farther westward expansion of SH but with a weaker strength. Conversely, the El Niño environment with stronger convections at lower latitudes leads to shrunken SH but with a greater strength. The influence of the seesaw mechanism appears to be modulated by global warming. The western North Pacific subtropical high strengthens overall under warming in both the La Niña and El Niño environments. This suggests that the weakening effect by drier tropics is largely offset by anomalous highs induced by a warming atmosphere. It is most remarkable that the highest SH strengths appear in a warmer El Niño environment. The finding implies that every new El Niño environment may experience the driest atmosphere ever in the subtropics under global warming. The value of this study lies in the fact that OLR effectively illustrates how the ENSO variation and global warming bring the zonally undulating strength of boreal-summer SH. Full article

As economically important sympatric species in the Northwest Pacific, the Japanese sardine (Sardinops melanostictus) and Chub mackerel (Scomber japonicus) exhibit significant biological interactions. Understanding the impact of interspecies competition on their habitat dynamics can provide crucial insights for the sustainable development and management of these interconnected species resources. This study utilizes fisheries data of S. melanostictus and S. japonicus from the Northwest Pacific, collected from June to November between 2017 and 2020. We integrated various environmental parameters, including temperature at different depths (0, 50, 100, 150, and 200 m), eddy kinetic energy (EKE), sea surface height (SSH), chlorophyll-a concentration (Chl-a), and the oceanic Niño index (ONI), to construct interspecific competition species distribution model (icSDM) for both species. We validated these models by overlaying the predicted habitats with fisheries data from 2021 and performing cross-validation to assess the models’ reliability. Furthermore, we conducted correlation analyses of the habitats of these two species to evaluate the impact of interspecies relationships on their habitat dynamics. The results indicate that, compared to single-species habitat models, the interspecific competition species distribution model (icSDM) for these two species exhibit a significantly higher explanatory power, with R² values increasing by up to 0.29; interspecific competition significantly influences the habitat dynamics of S. melanostictus and S. japonicus, strengthening the correlation between their habitat changes. This relationship exhibits a positive correlation at specific stages, with the highest correlations observed in June, July, and October, at 0.81, 0.80, and 0.88, respectively; interspecific competition also demonstrates stage-specific differences in its impact on the habitat dynamics of S. melanostictus and S. japonicus, with the most pronounced differences occurring in August and November. Compared to S. melanostictus, interspecific competition is more beneficial for the expansion of the optimal habitat (HIS ≥ 0.6) for S. japonicus and, to some extent, inhibits the habitat expansion of S. melanostictus. The variation in migratory routes and predatory interactions (with larger individuals of S. japonicus preying on smaller individuals of S. melanostictus) likely constitutes the primary factors contributing to these observed differences. Full article

Selenium (Se) is an important trace element in our body; however, food composition data remain limited due to analytical challenges and interferences. Seafood, abundant in Thailand, is recognized as a rich source of Se. This study aimed to expand knowledge on Se content in seafood prepared using traditional Thai cooking methods. Twenty seafood species were selected and prepared by boiling, frying, and grilling. Inductively Coupled Plasma–Triple Quadrupole–Mass Spectrometry (ICP-MS/MS) was used to analyze total Se contents in selected seafood species. Results revealed significant variation in Se content across species and cooking methods. The Indo-Pacific horseshoe crab showed the highest Se concentration, with fried samples reaching 193.9 μg/100 g. Se concentrations were in the range of 8.6–155.5 μg/100 g (fresh), 14.3–106.6 μg/100 g (boiled), 17.3–193.9 μg/100 g (fried), and 7.3–160.1 μg/100 g (grilled). Results found significant effects of species and cooking method on Se content (p < 0.05). Fried seafood exhibited the highest estimated marginal mean Se concentration (a 78.8 μg/100 g edible portion), significantly higher than other methods. True retention (%TR) of Se ranged from 40.4% to 100%, depending on species and method. Bigfin reef squid, wedge shell, and silver pomfret showed the highest %TR (100%), while splendid squid exhibited the lowest (52.5%). Significant interaction effects on %TR were also observed (p < 0.05). Fried seafood had the highest mean %TR (88.8%), followed by grilled (82.1%) and boiled (79.7%). These findings highlight the effects of both species and cooking method on Se retention, emphasizing the nutritional value of selected seafood in preserving bioavailable Se after cooking. Full article

The southern Great Xing’an Range is located in the overlap zone of the Paleo-Asian Ocean metallogenic domain and the Circum-Pacific metallogenic domain. It hosts numerous Sn-polymetallic deposits, such as Weilasituo, Bianjiadayuan, Huanggang, and Dajing, and witnessed multiple episodes of magmatism during the Late Mesozoic. The study area is situated within the Huanggangliang-Ganzhuermiao metallogenic belt in the southern Great Xing’an Range. The region has witnessed extensive magmatism, with Mesozoic magmatic activities being particularly closely linked to regional mineralization. We present petrographic, zircon U-Pb chronological, lithogeochemical, and Lu-Hf isotopic analyses of the Yexilinhundi granites. The results indicate that the granite porphyry and granodiorite were emplaced during the Late Jurassic. Both rocks exhibit high SiO₂, K₂O + Na₂O, differentiation index (DI), and 10,000 Ga/Al ratios, coupled with low MgO contents. They show distinct fractionation between light and heavy rare earth elements (LREEs and HREEs), exhibit Eu anomalies, and have low whole-rock zircon saturation temperatures (T_zr), collectively demonstrating characteristics of highly fractionated I-type granites. The εHf(t) values of the granites range from 0.600 to 9.14, with young two-stage model ages (T_DM2 = 616.0~1158 Ma), indicating that the magmatic source originated from partial melting of Mesoproterozoic-Neoproterozoic juvenile crust. This study proposes that the granites formed in a post-collisional/post-orogenic extensional setting associated with the subduction of the Mongol-Okhotsk Ocean, providing a scientific basis for understanding the relationship between the formation of Sn-polymetallic deposits and granitic magmatic evolution in the study area. Full article

The eastern North Pacific Ocean coastline (from the Salish Sea to the western Aleutian Islands) is highly glaciated with relic sediment deposits scattered throughout a highly contoured and variable bathymetry. Oceanographic conditions feature strong currents and tidal exchange. Sand wave fields are prominent features within these glaciated shorelines and provide critical habitat to sand lance (Ammodytes spp.). Despite an awareness of the importance of these benthic habitats, attributes related to their structure and characteristics remain undocumented. We explored the micro-bathymetric morphology of a subtidal sand wave field known to be a consistent habitat for sand lance. We calculated geomorphic attributes of the bedform habitat, analyzed sediment composition, and measured oceanographic properties of the associated water column. This feature has a streamlined teardrop form, tapered in the direction of the predominant tidal current. Consistent flow paths along the long axis contribute to well-defined and maintained bedform morphology and margin. Distinct patterns in amplitude and period of sand waves were documented. Strong tidal exchange has resulted in well-sorted medium-to-coarse-grained sediments with coarser sediments, including gravel and cobble, within wave troughs. Extensive mixing related to tidal currents results in a highly oxygenated water column, even to depths of 80 m. Our analysis provides unique insights into the physical characteristics that define high-quality habitat for these fish. Further work is needed to identify, enumerate, and map the presence and relative quality of these benthic habitats and to characterize the oceanographic properties that maintain these benthic habitats over time. Full article

This study presents a comprehensive taxonomic revision of the genus Dissomphalus Ashmead (Hymenoptera, Bethylidae) in the southwestern Pacific, focusing on specimens from Fiji and the Solomon Islands. Dissomphalus is the most species-rich genus within Bethylidae, with a global distribution, yet it remains poorly documented in the Oceanian region. We examined 151 male specimens collected in these islands. As a result, we describe 27 new species, 25 from Fiji and 2 from the Solomon Islands. Diagnostic morphological features, detailed illustrations, and an identification key for all species are provided. These findings substantially expand the known diversity of Dissomphalus in Oceania. Our results underscore the importance of taxonomic research in underexplored regions and highlight the potential for high levels of species endemism in island ecosystems. This revision contributes to a better understanding of the genus’ biogeographical distribution and provides essential tools for future biodiversity assessments and conservation efforts in the southwestern Pacific. Full article

We adapt previously developed conceptual and numerical models of ciguateric food chains on the Great Barrier Reef, Australia, to model the bioaccumulation of ciguatoxins (CTXs) in parrotfish, the simplest food chain with only two trophic levels. Our model indicates that relatively low (1 cell/cm²) densities of Gambierdiscus/Fukuyoa species (hereafter collectively referred to as Gambierdiscus) producing known concentrations of CTX are unlikely to be a risk of producing ciguateric fishes on the Great Barrier Reef unless CTX can accumulate and be retained in parrotfish over many months. Cell densities on turf algae equivalent to 10 Gambierdiscus/cm² producing known maximum concentrations of Pacific-CTX-4 (0.6 pg P-CTX-4/cell) are more difficult to assess but could be a risk. This cell density may be a higher risk for parrotfish than we previously suggested for production of ciguateric groupers (third-trophic-level predators) since second-trophic-level fishes can accumulate CTX loads without the subsequent losses that occur between trophic levels. Our analysis suggests that the ratios of parrotfish length-to-area grazed and weight-to-area grazed scale differently (allometrically), where the area grazed is a proxy for the number of Gambierdiscus consumed and hence proportional to toxin accumulation. Such scaling can help explain fish size–toxicity relationships within and between trophic levels for ciguateric fishes. Our modelling reveals that CTX bioaccumulates but does not necessarily biomagnify in food chains, with the relative enrichment and depletion rates of CTX varying with fish size and/or trophic level through an interplay of local and regional food chain influences. Our numerical model for the bioaccumulation and transfer of CTX across food chains helps conceptualize the development of ciguateric fishes by comparing scenarios that reveal limiting steps in producing ciguateric fish and focuses attention on the relative contributions from each part of the food chain rather than only on single components, such as CTX production. Full article

Acute hepatopancreatic necrosis disease (AHPND), caused by the bacterium Vibrio parahaemolyticus, is a major threat to global shrimp aquaculture. In this study, we evaluated the therapeutic effects of phage therapy in Litopenaeus vannamei challenged with AHPND-causing Vibrio parahaemolyticus. Phage application at various concentrations significantly improved shrimp survival, with the 1 ppm group demonstrating the highest survival rate. Enzymatic assays revealed that phage-treated shrimp exhibited enhanced immune enzyme activities, including acid phosphatase (ACP), alkaline phosphatase (AKP), and lysozyme (LZM). In addition, antioxidant defenses such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX), and total antioxidant capacity (T-AOC) significantly improved, accompanied by reduced malondialdehyde (MDA) levels. Serum biochemical analyses demonstrated marked improvements in lipid metabolism, particularly reductions in triglyceride (TG), total cholesterol (TC), and low-density lipoprotein (LDL), alongside higher levels of beneficial high-density lipoprotein (HDL). Transcriptomic analysis identified 2274 differentially expressed genes (DEGs), notably enriched in pathways involving fatty acid metabolism, peroxisome functions, lysosomes, and Toll-like receptor (TLR) signaling. Specifically, phage treatment upregulated immune and metabolic regulatory genes, including Toll-like receptor 4 (TLR4), myeloid differentiation primary response protein 88 (MYD88), interleukin-1β (IL-1β), nuclear factor erythroid 2-related factor 2 (Nrf2), and peroxisome proliferator-activated receptor (PPAR), indicating activation of innate immunity and antioxidant defense pathways. These findings suggest that phage therapy induces protective immunometabolic adaptations beyond its direct antibacterial effects, thereby providing an ecologically sustainable alternative to antibiotics for managing bacterial diseases in shrimp aquaculture. Full article