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Antihypertensive Effects of Corn Silk Extract and Its Novel Bioactive Constituent in Spontaneously Hypertensive Rats: The Involvement of Angiotensin-Converting Enzyme Inhibition

1
Graduate Institute of Chinese Medicine, China Medical University, Taichung 40402, Taiwan
2
Graduate Institute of Acupuncture Science, China Medical University, Taichung 40402, Taiwan
3
Department of Chinese Medicine, China Medical University Hospital, Taichung 40447, Taiwan
4
Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan
5
Department of Microbiology and Immunology, China Medical University, Taichung 40402, Taiwan
6
Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan
*
Authors to whom correspondence should be addressed.
Molecules 2019, 24(10), 1886; https://doi.org/10.3390/molecules24101886
Received: 10 April 2019 / Revised: 8 May 2019 / Accepted: 14 May 2019 / Published: 16 May 2019
(This article belongs to the Section Natural Products Chemistry)
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Abstract

Corn silk tea has been used in folk medicine for anti-hypertensive healthcare. Angiotensin-converting enzyme (ACE) plays a crucial role on the homeostasis of blood pressure. However, effects of corn silk tea on ACE activity and the presence of ACE inhibitory constituents in corn silk are still unknown. Here we applied proteomics and bioinformatics approaches to identify corn silk bioactive peptides (CSBps) that target ACE from the boiling water extract of corn silk (CSE). CSE significantly reduced systolic blood pressure (SBP) levels in spontaneously hypertensive rats and inhibited the ACE activity. By proteomics coupled with bioinformatics analyses, we identified a novel ACE inhibitory peptide CSBp5 in CSE. CSBp5 significantly inhibited the ACE activity and decreased SBP levels in a dose-dependent manner. Docking analysis showed that CSBp5 occupied the substrate-binding channel of ACE and interacted with ACE via hydrogen bonds. In conclusion, we identified that CSE exhibited anti-hypertensive effects in SHRs via the inhibition of ACE, the target of most anti-hypertensive drugs. In addition, an ACE inhibitory phytopeptide CSBp5 that decreased SBP levels in rats was newly identified. Our findings supported the ethnomedical use of corn silk tea on hypertension. Moreover, the identification of ACE inhibitory phytopeptide in corn silk further strengthened our findings. View Full-Text
Keywords: corn silk; hypertension; angiotensin-converting enzyme; bioactive phytopeptide corn silk; hypertension; angiotensin-converting enzyme; bioactive phytopeptide
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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MDPI and ACS Style

Li, C.-C.; Lee, Y.-C.; Lo, H.-Y.; Huang, Y.-W.; Hsiang, C.-Y.; Ho, T.-Y. Antihypertensive Effects of Corn Silk Extract and Its Novel Bioactive Constituent in Spontaneously Hypertensive Rats: The Involvement of Angiotensin-Converting Enzyme Inhibition. Molecules 2019, 24, 1886.

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