Next Article in Journal
Metal Chelating, Inhibitory DNA Damage, and Anti-Inflammatory Activities of Phenolics from Rambutan (Nephelium lappaceum) Peel and the Quantifications of Geraniin and Corilagin
Previous Article in Journal
Chemometric Comparison and Classification of Some Essential Oils Extracted from Plants Belonging to Apiaceae and Lamiaceae Families Based on Their Chemical Composition and Biological Activities
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 23
Issue 9
10.3390/molecules23092262
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Conformationally Restricted Peptides from Rice Proteins Elicit Antibodies That Recognize the Corresponding Native Protein in ELISA Assays

by
Nubia F. Barrera
1,2,
Luz M. Melgarejo
3,
Maribel Cruz-Gallego
4,
Lina Jimena Cortés
5,
Fanny Guzmán
5 and
Julio C. Calvo
2,*
1
Doctorado en Biotecnología, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Edificio 224, Bogotá 110111, Colombia
2
Grupo Proteoma UD, Facultad de Ciencias y Educación, Universidad Distrital Francisco José de Caldas, Carrera 4 No. 26B-54, Bogotá 110111, Colombia
3
Laboratorio de Fisiología y Bioquímica Vegetal, Departamento de Biología, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Edificio 421, Bogotá 110111, Colombia
4
Centro Internacional de Agricultura Tropical, CIAT, Fondo Latinoamericano para Arroz de Riego, FLAR, Palmira, Valle 763537, Colombia
5
Núcleo de Biotecnología de Curauma, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, Chile
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(9), 2262; https://doi.org/10.3390/molecules23092262
Submission received: 7 August 2018 / Revised: 29 August 2018 / Accepted: 1 September 2018 / Published: 5 September 2018
Browse Figures
Versions Notes

Abstract

The rice hoja blanca virus (RHBV), transmitted by the planthopper insect Tagosodes orizicolus, is a disease that attacks rice and generates significant production losses in Colombia. Fedearroz 2000 and Colombia I commercial rice varieties, which have different resistance levels to the disease, were selected in this study. To identify proteins associated to the insect and virus signaling, a comparative proteomics study was performed. By comparing proteomic profiles, between virus-infected and control group plants in two-dimensional electrophoresis, proteins exhibiting significant changes in abundance were found. In another test, peptide dendrimers containing sequences conformationally restricted to α-helix from four of those rice proteins were synthesized. In the experiment, sera from mice inoculated with peptide dendrimers could recognize the corresponding native protein in ELISA assays. Reported comparative proteomic results provide new insights into the molecular mechanisms of plant response to the RHBV and comprehensive tools for the analysis of new crop varieties. Besides, results from conformational peptide dendrimer approach are promising and show that it is feasible to detect proteins as markers, and may have biological applications by decreasing the susceptibility to proteolytic degradation.
Keywords: plant proteomics; RHBV; rice hoja blanca virus; Colombian rice varieties; peptide dendrimers; helix mimetics plant proteomics; RHBV; rice hoja blanca virus; Colombian rice varieties; peptide dendrimers; helix mimetics

Share and Cite

MDPI and ACS Style

Barrera, N.F.; Melgarejo, L.M.; Cruz-Gallego, M.; Cortés, L.J.; Guzmán, F.; Calvo, J.C. Conformationally Restricted Peptides from Rice Proteins Elicit Antibodies That Recognize the Corresponding Native Protein in ELISA Assays. Molecules 2018, 23, 2262. https://doi.org/10.3390/molecules23092262

AMA Style

Barrera NF, Melgarejo LM, Cruz-Gallego M, Cortés LJ, Guzmán F, Calvo JC. Conformationally Restricted Peptides from Rice Proteins Elicit Antibodies That Recognize the Corresponding Native Protein in ELISA Assays. Molecules. 2018; 23(9):2262. https://doi.org/10.3390/molecules23092262

Chicago/Turabian Style

Barrera, Nubia F., Luz M. Melgarejo, Maribel Cruz-Gallego, Lina Jimena Cortés, Fanny Guzmán, and Julio C. Calvo. 2018. "Conformationally Restricted Peptides from Rice Proteins Elicit Antibodies That Recognize the Corresponding Native Protein in ELISA Assays" Molecules 23, no. 9: 2262. https://doi.org/10.3390/molecules23092262

APA Style

Barrera, N. F., Melgarejo, L. M., Cruz-Gallego, M., Cortés, L. J., Guzmán, F., & Calvo, J. C. (2018). Conformationally Restricted Peptides from Rice Proteins Elicit Antibodies That Recognize the Corresponding Native Protein in ELISA Assays. Molecules, 23(9), 2262. https://doi.org/10.3390/molecules23092262

Article Metrics

Back to TopTop