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Isoflavones Production and Possible Mechanism of Their Exudation in Genista tinctoria L. Suspension Culture after Treatment with Vanadium Compounds

1
Department of Botany and Plant Physiology, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague, Czech Republic
2
Department of Pharmacognosy, Faculty of Pharmacy, Charles University, 500 02 Hradec Králové, Czech Republic
*
Authors to whom correspondence should be addressed.
Molecules 2018, 23(7), 1619; https://doi.org/10.3390/molecules23071619
Received: 1 June 2018 / Revised: 28 June 2018 / Accepted: 29 June 2018 / Published: 3 July 2018
(This article belongs to the Section Natural Products Chemistry)
The family Fabaceae traditionally serves as a food and herbal remedies source. Certain plants serve for treatment of menopausal symptoms based on a presence of typical secondary metabolites, isoflavones. Beside soybean and clovers, other plants or cultures in vitro can produce these molecules. A cultivation in vitro can be enhanced by elicitation that stimulates metabolites biosynthesis via stress reaction. Vanadium compounds have been already described as potential elicitors, and the aim of this study was to determine the impact of NH4VO3 and VOSO4 solutions on isoflavones production in Genista tinctoria L. cell cultures. The significant increase of isoflavones content, such as genistin, genistein, or formononetin, was measured in a nutrient medium or dry mass after NH4VO3 treatment for 24 or 48 h. The possible transport mechanism of isoflavones release as a result of elicitation was further evaluated. An incubation with different transport inhibitors prior to elicitation took effect on isoflavones content in the medium. However, there was a non-ended result for particular metabolites such as genistein and daidzein, where ATP-binding cassette (ABC) or, alternatively, multidrug and toxin extrusion (MATE) proteins can participate. Possible elicitation by some inhibitors was discussed as a result of their pleiotropic effect. Despite this outcome, the determination of the transport mechanism is an important step for identification of the specific transporter. View Full-Text
Keywords: heavy metals; Dyer’s Greenweed; elicitation; plasma membrane transport heavy metals; Dyer’s Greenweed; elicitation; plasma membrane transport
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MDPI and ACS Style

Skalicky, M.; Kubes, J.; Hejnak, V.; Tumova, L.; Martinkova, J.; Martin, J.; Hnilickova, H. Isoflavones Production and Possible Mechanism of Their Exudation in Genista tinctoria L. Suspension Culture after Treatment with Vanadium Compounds. Molecules 2018, 23, 1619.

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