Chalcone Derivatives Enhance ATP-Binding Cassette Transporters A1 in Human THP-1 Macrophages
AbstractAtherosclerosis is a process of imbalanced lipid metabolism in the vascular walls. The underlying pathology mainly involves the deposition of oxidized lipids in the endothelium and the accumulation of cholesterol in macrophages. Macrophages export excessive cholesterol (cholesterol efflux) through ATP-binding cassette transporter A1 (ABCA1) to counter the progression of atherosclerosis. We synthesized novel chalcone derivatives and assessed their effects and the underlying mechanisms on ABCA1 expression in macrophages. Human THP-1 macrophages were treated with synthetic chalcone derivatives for 24 h. In Western blot and flow cytometry analyses, a chalcone derivative, (E)-1-(3,4-diisopropoxyphenyl)-3-(4-isopropoxy-3-methoxyphenyl)prop- 2-en-1-one (1m), was observed to significantly enhance ABCA1 protein expression in THP-1 cells (10 µM, 24 h). Levels of mRNA of ABCA1 and liver X receptor alpha (LXRα) were quantified using a real-time quantitative polymerase chain reaction technique and were found to be significantly increased after treatment with the novel chalcone derivative 1m. Several microRNAs, including miR155, miR758, miR10b, miR145, miR33, and miR106b, which functionally inhibit ABCA1 expression were suppressed after treatment with 1m. Collectively, 1m increases ABCA1 expression in human THP-1 macrophages. The mechanisms involve the activation of the LXRα-ABCA1 pathway and suppression of certain microRNAs that regulate ABCA1 expression. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 385 KB)
Share & Cite This Article
Teng, I.-J.; Tsai, M.-C.; Shih, S.-F.; Tsuei, B.-F.; Chang, H.; Chuang, Y.-P.; Lin, C.-S.; Chern, C.-Y.; Chen, S.-J. Chalcone Derivatives Enhance ATP-Binding Cassette Transporters A1 in Human THP-1 Macrophages. Molecules 2018, 23, 1620.
Teng I-J, Tsai M-C, Shih S-F, Tsuei B-F, Chang H, Chuang Y-P, Lin C-S, Chern C-Y, Chen S-J. Chalcone Derivatives Enhance ATP-Binding Cassette Transporters A1 in Human THP-1 Macrophages. Molecules. 2018; 23(7):1620.Chicago/Turabian Style
Teng, I-Jou; Tsai, Min-Chien; Shih, Shao-Fu; Tsuei, Bi-Feng; Chang, Hsin; Chuang, Yi-Ping; Lin, Chin-Sheng; Chern, Ching-Yuh; Chen, Sy-Jou. 2018. "Chalcone Derivatives Enhance ATP-Binding Cassette Transporters A1 in Human THP-1 Macrophages." Molecules 23, no. 7: 1620.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.