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Molecules 2018, 23(5), 1050; https://doi.org/10.3390/molecules23051050

Effective Saccharification of Corn Stover Using Low-Liquid Aqueous Ammonia Pretreatment and Enzymatic Hydrolysis

1
Faculty of Natural Sciences, Thu Dau Mot University, Thu Dau Mot City 820000, Binh Duong Province, Vietnam
2
Department of Materials Science and Chemical Engineering, Hanyang University, Ansan, Gyeonggi-do 15588, Korea
*
Author to whom correspondence should be addressed.
Received: 27 March 2018 / Revised: 22 April 2018 / Accepted: 28 April 2018 / Published: 1 May 2018
(This article belongs to the Special Issue Efficient Technology for the Pretreatment of Biomass)
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Abstract

Low-liquid aqueous ammonia (LLAA) pretreatment using aqueous ammonia was investigated to enhance enzymatic saccharification of corn stover. In this method, ground corn stover was simply contacted with aqueous ammonia mist (ammoniation step), followed by pretreatment at elevated temperature (90–150 °C) for an extended period (24–120 h) at different solid/liquid (S/L) ratios (0.29, 0.47 or 0.67), termed a pretreatment step. After that, excess (unreacted) ammonia was removed by evaporation, and the pretreated material was immediately saccharified by an enzyme without a washing step. The effects of key reaction parameters on both glucan digestibility and XMG digestibility were evaluated by analysis of variance (ANOVA). Under the best pretreatment conditions [S/L = 0.47, 0.16 (g NH3)/(g biomass), 90 °C, 24 h], LLAA pretreatment enhanced enzymatic digestibility from 23.1% for glucan and 11.3% for XMG (xylan + galactan + mannan) of untreated corn stover to 91.8% for glucan and 72.6% for XMG in pretreated solid. View Full-Text
Keywords: aqueous ammonia; alkaline pretreatment; enzymatic digestibility; lignocellulosic biomass; cellulosic sugar aqueous ammonia; alkaline pretreatment; enzymatic digestibility; lignocellulosic biomass; cellulosic sugar
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Truong, N.P.V.; Kim, T.H. Effective Saccharification of Corn Stover Using Low-Liquid Aqueous Ammonia Pretreatment and Enzymatic Hydrolysis. Molecules 2018, 23, 1050.

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