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Comparison of Serum Metabolite Changes of Radiated Mice Administered with Panax quinquefolium from Different Cultivation Regions Using UPLC-Q/TOF-MS Based Metabolomic Approach

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2018, 23(5), 1014; https://doi.org/10.3390/molecules23051014
Received: 10 April 2018 / Revised: 22 April 2018 / Accepted: 24 April 2018 / Published: 26 April 2018
Chemometric analysis of bioactive compounds revealed that American ginsengs (AGs) from different cultivation regions of China had a difference in quality, which indicates their possible pharmacological difference. A UPLC-Q/TOF-MS-based untargeted metabolomic approach was used to uncover serum metabolite changes in radiated mice pre-administered with AG root decoctions from seven cultivation regions and to further assess their quality difference. OPLS-DA revealed that 51 metabolites (ESI) and 110 (ESI+) were differentially expressed in sera between the control and the radiated model mice. Heatmap analysis further revealed that AG could not reverse most of these radiation-altered metabolites, which indicates dietary supplement of AG before cobalt radiation had the weak potential to mediate serum metabolites that were altered by the sub-lethal high dose radiation. In addition, 83 (ESI) and 244 (ESI+) AG altered metabolites were detected in radiated mice under radiation exposure. Both OPLS-DA on serum metabolomes and heatmap analysis on discriminant metabolites showed that AGs from different cultivation regions differentially influenced metabolic alterations in radiated mice, which indicates AGs from different cultivation regions showed the pharmacological difference in modulation of metabolite changes. AGs from Shandong, Shanxi, and Beijing provinces had more similar pharmacological effects than AGs from USA, Canada, Jilin, and Heilongjiang. Finally, 28 important potential biomarkers were annotated and assigned onto three metabolic pathways including lipid, amino acid, and energy metabolisms. View Full-Text
Keywords: Panax quinquefolium; cultivation region; cobalt radiation; serum metabolomics; UPLC/Q-TOF-MS Panax quinquefolium; cultivation region; cobalt radiation; serum metabolomics; UPLC/Q-TOF-MS
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MDPI and ACS Style

Qin, Z.; Jia, C.; Liao, D.; Chen, X.; Li, X. Comparison of Serum Metabolite Changes of Radiated Mice Administered with Panax quinquefolium from Different Cultivation Regions Using UPLC-Q/TOF-MS Based Metabolomic Approach. Molecules 2018, 23, 1014. https://doi.org/10.3390/molecules23051014

AMA Style

Qin Z, Jia C, Liao D, Chen X, Li X. Comparison of Serum Metabolite Changes of Radiated Mice Administered with Panax quinquefolium from Different Cultivation Regions Using UPLC-Q/TOF-MS Based Metabolomic Approach. Molecules. 2018; 23(5):1014. https://doi.org/10.3390/molecules23051014

Chicago/Turabian Style

Qin, Zhenxian; Jia, Chan; Liao, Dengqun; Chen, Xiaofang; Li, Xian’en. 2018. "Comparison of Serum Metabolite Changes of Radiated Mice Administered with Panax quinquefolium from Different Cultivation Regions Using UPLC-Q/TOF-MS Based Metabolomic Approach" Molecules 23, no. 5: 1014. https://doi.org/10.3390/molecules23051014

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