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Molecules 2018, 23(5), 1015; https://doi.org/10.3390/molecules23051015

Comparative Analysis of the Complete Chloroplast Genomes of Four Aconitum Medicinal Species

1
College of Horticulture and Landscape, Yunnan Agricultural University, Kunming 650201, China
2
Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
3
Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
*
Authors to whom correspondence should be addressed.
Received: 8 April 2018 / Revised: 23 April 2018 / Accepted: 24 April 2018 / Published: 26 April 2018
(This article belongs to the Section Molecular Diversity)
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Abstract

Aconitum (Ranunculaceae) consists of approximately 400 species distributed in the temperate regions of the northern hemisphere. Many species are well-known herbs, mainly used for analgesia and anti-inflammatory purposes. This genus is well represented in China and has gained widespread attention for its toxicity and detoxification properties. In southwestern China, several Aconitum species, called ‘Dula’ in the Yi Nationality, were often used to control the poisonous effects of other Aconitum plants. In this study, the complete chloroplast (cp) genomes of these species were determined for the first time through Illumina paired-end sequencing. Our results indicate that their cp genomes ranged from 151,214 bp (A. episcopale) to 155,769 bp (A. delavayi) in length. A total of 111–112 unique genes were identified, including 85 protein-coding genes, 36–37 tRNA genes and eight ribosomal RNA genes (rRNA). We also analyzed codon usage, IR expansion or contraction and simple sequence repeats in the cp genomes. Eight variable regions were identified and these may potentially be useful as specific DNA barcodes for species identification of Aconitum. Phylogenetic analysis revealed that all five studied species formed a new clade and were resolved with 100% bootstrap support. This study will provide genomic resources and potential plastid markers for DNA barcoding, further taxonomy and germplasm exploration of Aconitum. View Full-Text
Keywords: ranunculaceae; ‘Dula’; comparative genomics; herbal medicine; phylogenetic analysis ranunculaceae; ‘Dula’; comparative genomics; herbal medicine; phylogenetic analysis
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Meng, J.; Li, X.; Li, H.; Yang, J.; Wang, H.; He, J. Comparative Analysis of the Complete Chloroplast Genomes of Four Aconitum Medicinal Species. Molecules 2018, 23, 1015.

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