Next Article in Journal
Discovery of a Manduca sexta Allatotropin Antagonist from a Manduca sexta Allatotropin Receptor Homology Model
Next Article in Special Issue
Optimization of the Ultrasonic-Assisted Extraction of Phenolic Compounds from Oryza Sativa L. ‘Violet Nori’ and Determination of the Antioxidant Properties of its Caryopses and Leaves
Previous Article in Journal
Tryptophan-Rich and Proline-Rich Antimicrobial Peptides
Previous Article in Special Issue
Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle
Molecules 2018, 23(4), 816; https://doi.org/10.3390/molecules23040816

Antioxidant Activities of Phenolic Metabolites from Flemingia philippinensis Merr. et Rolfe and Their Application to DNA Damage Protection

1
Division of Applied Life Science (BK21 plus), IALS, Gyeongsang National University, Jinju 52828, Korea
2
College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China
*
Author to whom correspondence should be addressed.
Received: 11 March 2018 / Revised: 31 March 2018 / Accepted: 2 April 2018 / Published: 2 April 2018
(This article belongs to the Special Issue The Antioxidant Capacities of Natural Products)
Full-Text   |   PDF [2702 KB, uploaded 3 May 2018]   |  

Abstract

F. philippinensis Merr. et Rolfe has been cultivated on a large scale and is widely consumed by local inhabitants as an important nutraceutical, especially against rheumatism which has a deep connection with antioxidants. In this study, a total of 18 different phenolic metabolite compounds in F. philippinensis were isolated and identified, and evaluated for their antioxidant and DNA damage protection potential. The antioxidant activity of the 18 identified compounds was screened using DPPH, ORAC, hydroxyl and superoxide radical scavenging assays. The antioxidant potential of the compounds was found to differ by functionality and skeleton. However, most compounds showed a good antioxidant potential. In particular, seven of the identified compounds, namely, compounds 2, 3, 6, 10, 11, 15 and 16, showed significant protective effects on pBR322 plasmid DNA against the mutagenic and toxic effects of Fenton’s reaction. The most active compound, compound 2, displayed a dose-dependent DNA damage protection potential in the range of 7.5~60.0 μM. The DNA damage protective effect of the identified compounds was significantly correlated with the hydroxyl radical scavenging activity. Compounds that exhibited effective (IC50 = 5.4~12.5 μg/mL) hydroxyl radical scavenging activity were found to be the ones with higher DNA damage protection potential. View Full-Text
Keywords: Flemingia philippinensis; phenolic metabolites; electron spin resonance spectroscopy; radical scavenging activity; DNA damage protective effects Flemingia philippinensis; phenolic metabolites; electron spin resonance spectroscopy; radical scavenging activity; DNA damage protective effects
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Kim, J.Y.; Wang, Y.; Song, Y.H.; Uddin, Z.; Li, Z.P.; Ban, Y.J.; Park, K.H. Antioxidant Activities of Phenolic Metabolites from Flemingia philippinensis Merr. et Rolfe and Their Application to DNA Damage Protection. Molecules 2018, 23, 816.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top