Molecules 2018, 23(4), 816; https://doi.org/10.3390/molecules23040816
Antioxidant Activities of Phenolic Metabolites from Flemingia philippinensis Merr. et Rolfe and Their Application to DNA Damage Protection
Jeong Yoon Kim 1, Yan Wang 2
, Yeong Hun Song 1, Zia Uddin 1, Zuo Peng Li 1, Yeong Jun Ban 1 and Ki Hun Park 1,*
, Yeong Hun Song 1, Zia Uddin 1, Zuo Peng Li 1, Yeong Jun Ban 1 and Ki Hun Park 1,*
1
Division of Applied Life Science (BK21 plus), IALS, Gyeongsang National University, Jinju 52828, Korea
2
College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China
*
Author to whom correspondence should be addressed.
Received: 11 March 2018 / Revised: 31 March 2018 / Accepted: 2 April 2018 / Published: 2 April 2018
(This article belongs to the Special Issue The Antioxidant Capacities of Natural Products)
Abstract
F. philippinensis Merr. et Rolfe has been cultivated on a large scale and is widely consumed by local inhabitants as an important nutraceutical, especially against rheumatism which has a deep connection with antioxidants. In this study, a total of 18 different phenolic metabolite compounds in F. philippinensis were isolated and identified, and evaluated for their antioxidant and DNA damage protection potential. The antioxidant activity of the 18 identified compounds was screened using DPPH, ORAC, hydroxyl and superoxide radical scavenging assays. The antioxidant potential of the compounds was found to differ by functionality and skeleton. However, most compounds showed a good antioxidant potential. In particular, seven of the identified compounds, namely, compounds 2, 3, 6, 10, 11, 15 and 16, showed significant protective effects on pBR322 plasmid DNA against the mutagenic and toxic effects of Fenton’s reaction. The most active compound, compound 2, displayed a dose-dependent DNA damage protection potential in the range of 7.5~60.0 μM. The DNA damage protective effect of the identified compounds was significantly correlated with the hydroxyl radical scavenging activity. Compounds that exhibited effective (IC50 = 5.4~12.5 μg/mL) hydroxyl radical scavenging activity were found to be the ones with higher DNA damage protection potential. View Full-TextKeywords:
Flemingia philippinensis; phenolic metabolites; electron spin resonance spectroscopy; radical scavenging activity; DNA damage protective effects
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Kim, J.Y.; Wang, Y.; Song, Y.H.; Uddin, Z.; Li, Z.P.; Ban, Y.J.; Park, K.H. Antioxidant Activities of Phenolic Metabolites from Flemingia philippinensis Merr. et Rolfe and Their Application to DNA Damage Protection. Molecules 2018, 23, 816.
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