Next Article in Journal
Synthesis, Characterization, and Antifungal Activity of Novel Benzo[4,5]imidazo[1,2-d][1,2,4]triazine Derivatives
Previous Article in Journal
Investigating the Interaction of Ascorbic Acid with Anthocyanins and Pyranoanthocyanins
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle

PER, a Circadian Clock Component, Mediates the Suppression of MMP-1 Expression in HaCaT Keratinocytes by cAMP

Biospectrum Life Science Institute, A-1805, U-TOWER, 767, Sinsu-ro, Suji-gu 16827, Yongin-si, Gyeonggi-do, Korea
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(4), 745; https://doi.org/10.3390/molecules23040745
Received: 14 February 2018 / Revised: 9 March 2018 / Accepted: 19 March 2018 / Published: 23 March 2018
  |  
PDF [9588 KB, uploaded 3 May 2018]
  |  

Abstract

Skin circadian clock system responds to daily changes, thereby regulating skin functions. Exposure of the skin to UV irradiation induces the expression of matrix metalloproteinase-1 (MMP-1) and causes DNA damage. It has been reported both DNA repair and DNA replication are regulated by the circadian clock in mouse skin. However, the molecular link between circadian clock and MMP-1 has little been investigated. We found PERIOD protein, a morning clock component, represses the expression of MMP-1 in human keratinocytes by using a PER-knockdown strategy. Treatment with siPer3 alleviated the suppression of MMP-1 expression induced by forskolin. Results revealed PER3 suppresses the expression of MMP-1 via cAMP signaling pathway. Additionally, we screened for an activator of PER that could repress the expression of MMP-1 using HaCaT cell line containing PER promoter-luciferase reporter gene. Results showed Lespedeza capitate extract (LCE) increased PER promoter activity. LCE inhibited the expression of MMP-1 and its effect of LCE was abolished in knockdown of PER2 or PER3, demonstrating LCE can repress the expression of MMP-1 through PER. Since circadian clock component PER can regulate MMP-1 expression, it might be a new molecular mechanism to develop therapeutics to alleviate skin aging and skin cancer. View Full-Text
Keywords: skin circadian clock; PER; MMP-1; cAMP; Lespedeza capitata skin circadian clock; PER; MMP-1; cAMP; Lespedeza capitata
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Yeom, M.; Lee, H.; Shin, S.; Park, D.; Jung, E. PER, a Circadian Clock Component, Mediates the Suppression of MMP-1 Expression in HaCaT Keratinocytes by cAMP. Molecules 2018, 23, 745.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top