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Molecules 2018, 23(3), 691; https://doi.org/10.3390/molecules23030691

Efficient (3S)-Acetoin and (2S,3S)-2,3-Butanediol Production from meso-2,3-Butanediol Using Whole-Cell Biocatalysis

1
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, China
These authors contribute equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 16 February 2018 / Revised: 6 March 2018 / Accepted: 12 March 2018 / Published: 19 March 2018
(This article belongs to the Special Issue Frontier in Biocatalysis for Organic Synthesis)
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Abstract

(3S)-Acetoin and (2S,3S)-2,3-butanediol are important platform chemicals widely applied in the asymmetric synthesis of valuable chiral chemicals. However, their production by fermentative methods is difficult to perform. This study aimed to develop a whole-cell biocatalysis strategy for the production of (3S)-acetoin and (2S,3S)-2,3-butanediol from meso-2,3-butanediol. First, E. coli co-expressing (2R,3R)-2,3-butanediol dehydrogenase, NADH oxidase and Vitreoscilla hemoglobin was developed for (3S)-acetoin production from meso-2,3-butanediol. Maximum (3S)-acetoin concentration of 72.38 g/L with the stereoisomeric purity of 94.65% was achieved at 24 h under optimal conditions. Subsequently, we developed another biocatalyst co-expressing (2S,3S)-2,3-butanediol dehydrogenase and formate dehydrogenase for (2S,3S)-2,3-butanediol production from (3S)-acetoin. Synchronous catalysis together with two biocatalysts afforded 38.41 g/L of (2S,3S)-butanediol with stereoisomeric purity of 98.03% from 40 g/L meso-2,3-butanediol. These results exhibited the potential for (3S)-acetoin and (2S,3S)-butanediol production from meso-2,3-butanediol as a substrate via whole-cell biocatalysis. View Full-Text
Keywords: meso-2,3-butanediol; (3S)-acetoin; (2S,3S)-butanediol; Escherichia coli; coenzyme regeneration; whole-cell biocatalysis meso-2,3-butanediol; (3S)-acetoin; (2S,3S)-butanediol; Escherichia coli; coenzyme regeneration; whole-cell biocatalysis
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He, Y.; Chen, F.; Sun, M.; Gao, H.; Guo, Z.; Lin, H.; Chen, J.; Jin, W.; Yang, Y.; Zhang, L.; Yuan, J. Efficient (3S)-Acetoin and (2S,3S)-2,3-Butanediol Production from meso-2,3-Butanediol Using Whole-Cell Biocatalysis. Molecules 2018, 23, 691.

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