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Article

Inhibition of Cytochrome P450 Activities by Extracts of Hyptis verticillata Jacq.: Assessment for Potential HERB-Drug Interactions

1
Natural Products Institute, University of the West Indies, Mona, Kingston 7, Jamaica
2
Tropical Medicine Research Institute, University of the West Indies, Mona, Kingston 7, Jamaica
3
School of Life Sciences, Pharmacy & Chemistry, Kingston University, Kingston upon Thames, UK
4
College of Science, United Arab Emirates University, Al-Ain 009713, United Arab Emirates
5
Department of Chemistry, University of the West Indies, Mona, Kingston 7, Jamaica
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(2), 430; https://doi.org/10.3390/molecules23020430
Received: 4 December 2017 / Revised: 6 February 2018 / Accepted: 13 February 2018 / Published: 15 February 2018
Understanding the potential for adverse drug reactions (ADRs), from herb-drug interactions, is a key aspect of medicinal plant safety, with particular relevance for public health in countries where medicinal plant use is highly prevalent. We undertook an in-depth assessment of extracts of Hyptis verticillata Jacq., via its impact on activities of key cytochrome P450 (CYP) enzymes (CYPs 1A1, 1A2, 1B1, 3A4 and 2D6), its antioxidant properties (determined by DPPH assays) and chemical characterisation (using LC-MS). The dried plant aqueous extract demonstrated potent inhibition of the activities of CYPs 1A1 (7.6 µg/mL), 1A2 (1.9 µg/mL), 1B1 (9.4 µg/mL) and 3A4 (6.8 µg/mL). Further analysis of other crude extracts demonstrated potent inhibition of CYP1A2 activity for a dried plant ethanol extract (1.5 µg/mL), fresh plant ethanol extract (3.9 µg/mL), and moderate activity for a fresh plant aqueous extract (27.8 µg/mL). All four extracts demonstrated strong antioxidant activity, compared to the positive control (ascorbic acid, 1.3 µg/mL), with the dried plant ethanol extract being the most potent (1.6 µg/mL). Analysis of the dried plant aqueous extract confirmed the identity of seven phytochemicals, five lignans and two triterpenes. Individual screening of these phytochemicals against the activity of CYP1A2 identified yatein as a moderate inhibitor (71.9 μM), likely to contribute to the plant extract’s potent bioactivity. Further analysis on the impact of this plant on key drug metabolizing enzymes in vivo appears warranted for likely ADRs, as well as furthering development as a potential chemopreventive agent. View Full-Text
Keywords: cytochrome P450; Hyptis verticillata Jacq.; enzyme inhibition; herb-drug interaction; drug-herb interaction; adverse drug reaction; ADR; chemoprevention; antioxidant cytochrome P450; Hyptis verticillata Jacq.; enzyme inhibition; herb-drug interaction; drug-herb interaction; adverse drug reaction; ADR; chemoprevention; antioxidant
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MDPI and ACS Style

Picking, D.; Chambers, B.; Barker, J.; Shah, I.; Porter, R.; Naughton, D.P.; Delgoda, R. Inhibition of Cytochrome P450 Activities by Extracts of Hyptis verticillata Jacq.: Assessment for Potential HERB-Drug Interactions. Molecules 2018, 23, 430. https://doi.org/10.3390/molecules23020430

AMA Style

Picking D, Chambers B, Barker J, Shah I, Porter R, Naughton DP, Delgoda R. Inhibition of Cytochrome P450 Activities by Extracts of Hyptis verticillata Jacq.: Assessment for Potential HERB-Drug Interactions. Molecules. 2018; 23(2):430. https://doi.org/10.3390/molecules23020430

Chicago/Turabian Style

Picking, David, Bentley Chambers, James Barker, Iltaf Shah, Roy Porter, Declan P Naughton, and Rupika Delgoda. 2018. "Inhibition of Cytochrome P450 Activities by Extracts of Hyptis verticillata Jacq.: Assessment for Potential HERB-Drug Interactions" Molecules 23, no. 2: 430. https://doi.org/10.3390/molecules23020430

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